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Revision as of 08:14, 8 October 2016
![](https://static.igem.org/mediawiki/2016/b/b0/T--Technion_Israel--collaboration_cover.jpg)
iGEM - Peshawar
Guidance
At the beginning we contacted Peshawar iGEM group because we discovered that both projects deal with detection. When we realized that the Peshawar group is the first team to represent Pakistan in iGEM we decided to share with them the knowledge that was accumulated from past Technion iGEM teams. The first step was to open a joint Facebook group, so each team member from every group would be able to find his colleague on the opposite team. Later, we had Skype sessions regarding mathematical modeling, Human practices and Cloning.
![](http://freshdirtdesigns.com/blog/wp-content/uploads/2011/12/image_350x200_placeholder.png)
![](http://freshdirtdesigns.com/blog/wp-content/uploads/2011/12/image_350x200_placeholder.png)
Cloning
Peshawar had issues with the transformation of plasmids into competent cells.
They elaborated on the exact steps they did in the lab and we tried to debug their procedure.
We also shared with team Peshawar our protocol for chemical transformation(3) and the protocol for producing competent cells(4).
After following our instructions the Peshawar team managed to perform a successful transformation.
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![](https://static.igem.org/mediawiki/2016/5/5a/T--Technion_Israel--up_arrow.png)