Difference between revisions of "Team:Paris Saclay/Notebook/June/22"
(→Wednesday 22ond June) |
(→Lab work) |
||
| (8 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
| − | =Wednesday 22<sup> | + | =Wednesday 22<sup>nd</sup> June= |
| − | + | ||
| − | ==== | + | ====Heat-shock competent cells (cf. [[Team:Paris_Saclay/Experiments#HeatShockCompetent|protocol]])==== |
| − | '' | + | ''By Marion'' |
At 10am OD was 0.3. At 1pm OD was 0.46. At 3pm OD was 0.62. | At 10am OD was 0.3. At 1pm OD was 0.46. At 3pm OD was 0.62. | ||
| − | The solution was put on ice 10min (stop the OD). After, it was centrifuged 10min at 4°C at 3000rpm. The pellet was resuspended in 80mL ice-cold TB and incubated on ice for 10min. The solution was centrifuged again at 4°C at 3000rpm. The pellet was resuspended in 20mL ice-cold TB and DMSO was added at 7% of the final concentration (1.58mL of DMSO for 21mL of TB+cells). The solution was incubated on ice for 10min and then distributed in tubes, frozen in liquid nitrogen and stored at -70°C. | + | The solution was put on ice 10min (in order to stop the OD). After, it was centrifuged 10min at 4°C at 3000rpm. The pellet was resuspended in 80mL ice-cold TB and incubated on ice for 10min. The solution was centrifuged again at 4°C at 3000rpm. The pellet was resuspended in 20mL ice-cold TB and DMSO was added at 7% of the final concentration (1.58mL of DMSO for 21mL of TB+cells). The solution was incubated on ice for 10min and then distributed in tubes, frozen in liquid nitrogen and stored at -70°C. |
| − | + | ||
===Interlab study=== | ===Interlab study=== | ||
====Transformation==== | ====Transformation==== | ||
| − | '' | + | ''By Lea and Caroline'' |
| − | No colony grew on the Petri dish. Transformations have to be | + | No colony grew on the Petri dish. Transformations have to be done again. |
| − | + | The [[Team:Paris_Saclay/Experiments#ElectroCompetent|usual protocol]] was followed but this time 2µL of plasmids were taken, a new solution of antibiotic Cm was used and a positive control was added (a pSB1C3 plasmid). | |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:05, 9 October 2016
