Difference between revisions of "Team:Paris Saclay/Notebook/June/23"
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| − | {{Team: Paris_Saclay/notebook_header}} | + | {{Team:Paris_Saclay/notebook_header}} |
| − | = | + | =Thursday 23<sup>rd</sup> June= |
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| − | === | + | ====Heat-shock competent cells==== |
| − | + | ''By Caroline and Lea'' | |
| − | '' | + | |
| − | {{Team: Paris_Saclay/notebook_footer}} | + | The cells competency was checked by transforming cells [[Team:Paris_Saclay/Experiments#HeatShockCompetent|as usual ]] with two control plasmids: |
| + | *the Interlab study negative control | ||
| + | *pSB1C3 blue | ||
| + | |||
| + | ===Interlab study=== | ||
| + | ====Transformation results==== | ||
| + | ''By Caroline, Lea and Marion'' | ||
| + | |||
| + | Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely. | ||
| + | |||
| + | ====Transformed cell cultures==== | ||
| + | ''By Lea'' | ||
| + | |||
| + | One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony for each was taken into 3.5mL LB medium + Cm at 30µg/mL. | ||
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| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:06, 9 October 2016
