Difference between revisions of "Team:Paris Saclay/Notebook/June/23"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 23<sup>rd</sup> June= | =Thursday 23<sup>rd</sup> June= | ||
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| − | ==== | + | ====Heat-shock competent cells==== |
''By Caroline and Lea'' | ''By Caroline and Lea'' | ||
| − | + | The cells competency was checked by transforming cells [[Team:Paris_Saclay/Experiments#HeatShockCompetent|as usual ]] with two control plasmids: | |
| − | + | *the Interlab study negative control | |
| + | *pSB1C3 blue | ||
===Interlab study=== | ===Interlab study=== | ||
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''By Caroline, Lea and Marion'' | ''By Caroline, Lea and Marion'' | ||
| − | Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 | + | Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely. |
====Transformed cell cultures==== | ====Transformed cell cultures==== | ||
''By Lea'' | ''By Lea'' | ||
| − | + | One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony for each was taken into 3.5mL LB medium + Cm at 30µg/mL. | |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:06, 9 October 2016
