Difference between revisions of "Team:Paris Saclay/Notebook/June/30"
(→Digestion of the plasmids containing gBlocks) |
(→Lab work) |
||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 30<sup>th</sup> June= | =Thursday 30<sup>th</sup> June= | ||
| − | |||
===Visualization=== | ===Visualization=== | ||
| Line 7: | Line 6: | ||
''By Alice and Lea'' | ''By Alice and Lea'' | ||
| − | Plasmids pPS16_001-009 (except pPS16_004) were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|extraction protocol]]. Each plasmids were extracted from 6 different clones of bacteria. | + | Plasmids [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001-009]] (except pPS16_004) were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|extraction protocol]]. Each plasmids were extracted from 6 different clones of bacteria. |
| − | At the end, plasmids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) | + | At the end, plasmids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water). |
====Digestion of the plasmids containing gBlocks==== | ====Digestion of the plasmids containing gBlocks==== | ||
''By Alice and Léa'' | ''By Alice and Léa'' | ||
| − | After extraction, plasmids were [[Team:Paris_Saclay/Experiments#PlasmidDigestion|digested with EcoRI and HindIII | + | After extraction, plasmids were [[Team:Paris_Saclay/Experiments#PlasmidDigestion|digested]] with EcoRI and HindIII. |
{| class="wikitable" | {| class="wikitable" | ||
| Line 47: | Line 46: | ||
|2.7 and 1 | |2.7 and 1 | ||
|- | |- | ||
| − | |pPS16_002 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_002|pPS16_002]] |
|3.7 | |3.7 | ||
|2.7 and 1 | |2.7 and 1 | ||
|- | |- | ||
| − | |pPS16_003 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_003|pPS16_003]] |
|3.7 | |3.7 | ||
|2.7 and 1 | |2.7 and 1 | ||
|- | |- | ||
| − | |pPS16_005 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_005|pPS16_005]] |
|3.7 | |3.7 | ||
|2.7 and 1 | |2.7 and 1 | ||
|- | |- | ||
| − | |pPS16_006 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_006|pPS16_006]] |
|3.7 | |3.7 | ||
|2.7 and 1 | |2.7 and 1 | ||
|- | |- | ||
| − | |pPS16_007 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_007|pPS16_007]] |
|3.4 | |3.4 | ||
|2.7 and 0.7 | |2.7 and 0.7 | ||
|- | |- | ||
| − | |pPS16_008 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_008|pPS16_008]] |
|4 | |4 | ||
|2.7 and 1.3 | |2.7 and 1.3 | ||
|- | |- | ||
| − | |pPS16_009 | + | |[[Team:Paris_Saclay/Notebook/June/28#pPS16_009|pPS16_009]] |
|3.6 | |3.6 | ||
|2.7 and 0.9 | |2.7 and 0.9 | ||
| Line 77: | Line 76: | ||
===Biobrick characterization=== | ===Biobrick characterization=== | ||
| − | ==== | + | ====BL21 electrocompetent cells preparation and transformation==== |
''By Caroline and Charlene'' | ''By Caroline and Charlene'' | ||
| − | The | + | The [[Team:Paris_Saclay/Experiments#ElectroCompetent|usual protocol]] was used for transforming cells with K1372001, K1372001+pcl_TAA and the controls which were done following the same protocol but without plasmids. |
| − | K1372001 and the controls were streaked on LB + Cm (30µg/mL). | + | K1372001 and the controls were streaked on LB + Cm (30µg/mL). K1372001+pcl_TAA was streaked on LB + Cm (30µg/mL) + streptomicin (50µg/mL). |
| − | This time two different streaks were made for each plasmid | + | This time two different streaks were made for each plasmid, the first one using 50µL from the culture, the second one with 400µL concentrated by centrifugation. |
| − | The | + | The Petri dishes made on 29/06 were stored at 4ºC. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 13:52, 9 October 2016
