Difference between revisions of "Team:Paris Saclay/Notebook/June/30"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 30<sup>th</sup> June= | =Thursday 30<sup>th</sup> June= | ||
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===Visualization=== | ===Visualization=== | ||
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Plasmids [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001-009]] (except pPS16_004) were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|extraction protocol]]. Each plasmids were extracted from 6 different clones of bacteria. | Plasmids [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001-009]] (except pPS16_004) were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|extraction protocol]]. Each plasmids were extracted from 6 different clones of bacteria. | ||
| − | At the end, plasmids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water) | + | At the end, plasmids were resuspended in water/RNAse (5µL RNAse 10 mg/mL for 1 mL of water). |
====Digestion of the plasmids containing gBlocks==== | ====Digestion of the plasmids containing gBlocks==== | ||
''By Alice and Léa'' | ''By Alice and Léa'' | ||
| − | After extraction, plasmids were [[Team:Paris_Saclay/Experiments#PlasmidDigestion|digested with EcoRI and HindIII | + | After extraction, plasmids were [[Team:Paris_Saclay/Experiments#PlasmidDigestion|digested]] with EcoRI and HindIII. |
{| class="wikitable" | {| class="wikitable" | ||
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''By Caroline and Charlene'' | ''By Caroline and Charlene'' | ||
| − | The [[Team:Paris_Saclay/Experiments#ElectroCompetent|usual protocol]] was used for | + | The [[Team:Paris_Saclay/Experiments#ElectroCompetent|usual protocol]] was used for transforming cells with K1372001, K1372001+pcl_TAA and the controls which were done following the same protocol but without plasmids. |
K1372001 and the controls were streaked on LB + Cm (30µg/mL). K1372001+pcl_TAA was streaked on LB + Cm (30µg/mL) + streptomicin (50µg/mL). | K1372001 and the controls were streaked on LB + Cm (30µg/mL). K1372001+pcl_TAA was streaked on LB + Cm (30µg/mL) + streptomicin (50µg/mL). | ||
This time two different streaks were made for each plasmid, the first one using 50µL from the culture, the second one with 400µL concentrated by centrifugation. | This time two different streaks were made for each plasmid, the first one using 50µL from the culture, the second one with 400µL concentrated by centrifugation. | ||
Latest revision as of 13:52, 9 October 2016
