Difference between revisions of "Team:Paris Saclay/Notebook/July/22"
(→High Fidelity Q5 PCR of transformed DH5α with pPS16_004 and pPS16_007) |
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===Visualization=== | ===Visualization=== | ||
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====Low Fidelity Dreamtaq PCR of DH5α|pPS16_002==== | ====Low Fidelity Dreamtaq PCR of DH5α|pPS16_002==== | ||
''By Mathilde'' | ''By Mathilde'' | ||
| − | A DreamTaq PCR was made with [[Team:Paris_Saclay/Notebook/July/19#Visualization|DH5α|pPS16_002]] cultures following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] with Tm at | + | A DreamTaq PCR was made with [[Team:Paris_Saclay/Notebook/July/19#Visualization|DH5α|pPS16_002]] cultures following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] with Tm at 57°C. |
We divided up the PCR mix in 6 PCR tubes and added in each one a different clone from the transformed culture. | We divided up the PCR mix in 6 PCR tubes and added in each one a different clone from the transformed culture. | ||
| − | The picked colonies were | + | The picked colonies were spread on a Petri dish with LB + Ampicillin (50µg/mL) + X-Gal/IPTG (1/1000). |
Results : | Results : | ||
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|960 | |960 | ||
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The electropheresis on agarose gel showed absolutely no PCR products. | The electropheresis on agarose gel showed absolutely no PCR products. | ||
| − | pPS16_002 transformation from the 19/07/2016 | + | pPS16_002 transformation from the 19/07/2016 was spread (50µL) on two Petri dishes LB + Ampicillin (50µg/mL) + X-Gal/IPTG (1/1000). |
====High Fidelity Q5 PCR of transformed DH5α with pPS16_004 and pPS16_007 ==== | ====High Fidelity Q5 PCR of transformed DH5α with pPS16_004 and pPS16_007 ==== | ||
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| − | [[T-- | + | [[File:T--Paris_Saclay--160722_Visualisation_PCRpPS16_002_7.JPG|400px|thumb|right|Migration of pPS16_002 and pPS16_007]] |
====Glycerol stocks for DH5α transformed with pcl_TAA, pcl_TAG and pcl_Tq ==== | ====Glycerol stocks for DH5α transformed with pcl_TAA, pcl_TAG and pcl_Tq ==== | ||
Latest revision as of 15:56, 9 October 2016
