Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Wednesday 27<sup>th</sup> July= | =Wednesday 27<sup>th</sup> July= | ||
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===Visualization=== | ===Visualization=== | ||
| − | ======== | + | ====Transformation of DH5a cells ==== |
| − | ''By '' | + | ''By Léa '' |
| + | DH5a were transformed with pPS16_001, pPS16_002, pPS16_005, or pPS16_009 using the [[Team:Paris_Saclay/Experiments#HeatShockCompetent|usual protocol]]. | ||
| + | pPS16_002 ligation products of the 19th of July and the 27th of July were used. | ||
| + | pPS16_001, pPS16_005 and pPS16_009 ligation products of the 26th of July were used. | ||
| + | The Heat shock were performed at 42°C during 60 seconds. | ||
| + | |||
| + | After a one hour incubation, transformation products were streaked on Petri dishes containing Ampicilin, xGal and IPTG, and incubated at 37°C overnight. | ||
| + | |||
| + | ====gBlock 1.2 insertion in puc19==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | ||
===Biobrick Characterization=== | ===Biobrick Characterization=== | ||
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We obtained transformed bacteria for every conditions made yesterday (except for controls). | We obtained transformed bacteria for every conditions made yesterday (except for controls). | ||
| − | [[File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg| | + | [[File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_TAA and K1372001]] |
| − | [[File:T--Paris_Saclay--160711_characterization_K13-TAG.jpg| | + | [[File:T--Paris_Saclay--160711_characterization_K13-TAG.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_TAG and K1372001]] |
| − | [[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg| | + | [[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_Tq and K1372001]] |
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| + | <br clear=all> | ||
| − | We wanted to have glycerol stock of our transformed bacteria so we had to make a | + | We wanted to have glycerol stock of our transformed bacteria so we had to make a preculture. 3 clones of each conditions were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 16:10, 9 October 2016
