Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Wednesday 27<sup>th</sup> July= | =Wednesday 27<sup>th</sup> July= | ||
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===Visualization=== | ===Visualization=== | ||
====Transformation of DH5a cells ==== | ====Transformation of DH5a cells ==== | ||
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The Heat shock were performed at 42°C during 60 seconds. | The Heat shock were performed at 42°C during 60 seconds. | ||
| − | After a one hour incubation, transformation products were streaked on Petri dishes containing Ampicilin, | + | After a one hour incubation, transformation products were streaked on Petri dishes containing Ampicilin, xGal and IPTG, and incubated at 37°C overnight. |
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| + | ====gBlock 1.2 insertion in puc19==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | ||
===Biobrick Characterization=== | ===Biobrick Characterization=== | ||
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[[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_Tq and K1372001]] | [[File:T--Paris_Saclay--160711_characterization_K13-Tq.jpg|210px|thumb|right|Colonies of BL21 transformed with pcl_Tq and K1372001]] | ||
| − | <br clear=all> | + | <br clear=all> |
| − | We wanted to have glycerol stock of our transformed bacteria so we had to make a | + | We wanted to have glycerol stock of our transformed bacteria so we had to make a preculture. 3 clones of each conditions were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 16:10, 9 October 2016
