Difference between revisions of "Team:Paris Saclay/Notebook/July/28"
(→Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009) |
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Thursday 28<sup>th</sup> July= | = Thursday 28<sup>th</sup> July= | ||
| − | + | ||
===Visualization=== | ===Visualization=== | ||
====Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009==== | ====Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009==== | ||
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Thus, the PCR mix was done for 24 tubes following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]]. | Thus, the PCR mix was done for 24 tubes following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]]. | ||
| − | Each clone was taken off from the | + | Each clone was taken off from the Petri dish (27/07) soaked in a PCR mix and finally spread on a Petri dish (LB+AMP+X-Gal+IPTG. |
PCR was done with a Tm at 57°C. | PCR was done with a Tm at 57°C. | ||
| − | Each PCR product was | + | Each PCR product was put on agarose gel to migrate. |
| − | 10µL of blue ladder was used | + | 10µL of blue ladder was used. |
PCR products expected were : | PCR products expected were : | ||
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[[File:T--Paris_Saclay--160728_visualization_PCR_Gblocks.jpeg|400px|thumb|right|Migration of pPS16_001 (Gblock 1.1) and pPS16_002 (Gblock 1.2)]] | [[File:T--Paris_Saclay--160728_visualization_PCR_Gblocks.jpeg|400px|thumb|right|Migration of pPS16_001 (Gblock 1.1) and pPS16_002 (Gblock 1.2)]] | ||
| + | [[File:T--Paris_Saclay--160728_visualization_PCR_Gblocks_GFP.jpeg|400px|thumb|right|Migration of pPS16_005 (Gblock 3.1) and pPS16_009 (GFP)]] | ||
====Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009==== | ====Low Fidelity DreamTaqPCR of DH5a|pPS16_001, DH5a|pPS16_002, DH5a|pPS16_005 and DH5a|pPS16_009==== | ||
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The exact same experiment as previously in the day was made, but with 6 different clones for each cultures. | The exact same experiment as previously in the day was made, but with 6 different clones for each cultures. | ||
| − | Each clone was taken off from the | + | Each clone was taken off from the Petri dish (27/07) soaked in a PCR mix and finally spread on a Petri dish (LB+AMP+X-Gal+IPTG. Each clone was also put in culture in 1,5mL of LB and put in incubation at 37°c, 180 rpm ON. |
| + | |||
| + | |||
| + | [[File:T--Paris_Saclay--160809_visualization3_-_Copie.jpeg|400px|thumb|right|Migration of Gblock 3.1 and GFP]] | ||
| + | [[File:T--Paris_Saclay--160809_visualization1.jpeg|400px|thumb|right|Migration of Gblock 1.1 and 1.2]] | ||
| + | [[File:T--Paris_Saclay--160809_visualization2.jpeg|200px|thumb|right|Migration of Gblock 1.1 and 1.2]] | ||
===Biobrick Characterization=== | ===Biobrick Characterization=== | ||
Latest revision as of 16:11, 9 October 2016
