Difference between revisions of "Team:Paris Saclay/Notebook/August/1"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Monday 1<sup>st</sup> August= | = Monday 1<sup>st</sup> August= | ||
| − | + | ||
===Visualization=== | ===Visualization=== | ||
====New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19==== | ====New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19==== | ||
''By Léa and Charlène'' | ''By Léa and Charlène'' | ||
| − | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | + | The insertion of these gblocks was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. |
====Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19==== | ====Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19==== | ||
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gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
| + | For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes. | ||
| + | |||
| + | ====gBlock 3.1 insertion in puc19==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | ||
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| + | ==== DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 streak to recover colonies ==== | ||
| + | ''By Caroline'' | ||
| + | Checked DH5alp transformed with pPS16_003, pPS16_004, pPS16_006 and pPS16_007 were streaked on Pteri dishes containing LB + Amp (50µg/mL) + IPTG (0.1 µL/mL) + Xgal (0.25µL/mL) from glycerol cultures. | ||
Latest revision as of 16:14, 9 October 2016
