Difference between revisions of "Team:Paris Saclay/Notebook/August/1"
(→DH5alp|pPS16_003, DH5alp|pPS16_003, DH5alp|pPS16_003 and DH5alp|pPS16_003 streak to recover colonies) |
(→Lab work) |
||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Monday 1<sup>st</sup> August= | = Monday 1<sup>st</sup> August= | ||
| − | + | ||
===Visualization=== | ===Visualization=== | ||
====New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19==== | ====New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19==== | ||
''By Léa and Charlène'' | ''By Léa and Charlène'' | ||
| − | The insertion was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. | + | The insertion of these gblocks was carried out following the usual [[Team:Paris_Saclay/Experiments#Ligation|protocol]]. |
====Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19==== | ====Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19==== | ||
| Line 12: | Line 12: | ||
gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. | ||
| + | For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes. | ||
====gBlock 3.1 insertion in puc19==== | ====gBlock 3.1 insertion in puc19==== | ||
Latest revision as of 16:14, 9 October 2016
