Difference between revisions of "Team:Paris Saclay/Notebook/August/2"
(→DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
(→Lab work) |
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
| − | = | + | = Tuesday 2<sup>nd</sup> August= |
| − | + | ||
===Visualization=== | ===Visualization=== | ||
| − | ==== | + | ==== DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015 ==== |
''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ||
| − | A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments# | + | A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]] and using the puc19 [[Team:Paris_Saclay/Experiments#primers|primers]]. 6 clones for each transformations were tested. |
| + | |||
| + | Each clone was spread and put at 37°C. | ||
| + | We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm). | ||
| + | |||
| + | [[File:T--Paris Saclay--160802 gel pcr 1.jpg|400px|thumb|right|Results]] | ||
| + | [[File:T--Paris Saclay--160802 gel pcr 2.jpg|400px|thumb|right|Results]] | ||
| + | [[File:T--Paris Saclay--160802 gel pcr 3.jpg|400px|thumb|right|Results]] | ||
==== Sequencing of the gBlock 4.2 ==== | ==== Sequencing of the gBlock 4.2 ==== | ||
''By Caroline'' | ''By Caroline'' | ||
| − | The PCR products | + | The PCR products obtained with Q5 on pPS16_008 were sent for sequencing. |
| + | |||
| + | ==== Culture of DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006, DH5alp|pPS16_007 and DH5alp|pPS16_008 ==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | One colonie from each transformation was put into LB + Amp (50µg/mL) and incubated overnight at 37°C and 180rpm. | ||
| + | |||
| + | ===Interlab Study=== | ||
| + | ==== Device 1 transformation ==== | ||
| + | ''By Caroline'' | ||
| + | |||
| + | The device 1 was transformed in DH5alp following the usual Heat-shock [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]. The transformations were streaked on Petri dishes LB+Amp(50µg/mL)+IPTG(0.1µL/mL)+XGal(0.25µL/mL) and incubated overnight at 37°C. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 16:16, 9 October 2016
