Difference between revisions of "Team:Paris Saclay/Notebook/August/8"
(→Liquid cultures of bacteria containing plasmid to extract) |
(→Lab work) |
||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
=Monday 8<sup>th</sup> August= | =Monday 8<sup>th</sup> August= | ||
| − | + | ||
===Visualization=== | ===Visualization=== | ||
| Line 8: | Line 8: | ||
The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#phusion|protocol]] adapted to 50µL and with a TM at 72°C. The specific [[Team:Paris_Saclay/Experiments#primers|primers]] for each parts were used. The products were put for migration on a 0.8%agarose gel with BET. No amplification were observed: that was probably due to the fact that the plasmid extractions made on the 3/08/2016 were eluted with water which is not working with this plasmid extraction kit. | The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#phusion|protocol]] adapted to 50µL and with a TM at 72°C. The specific [[Team:Paris_Saclay/Experiments#primers|primers]] for each parts were used. The products were put for migration on a 0.8%agarose gel with BET. No amplification were observed: that was probably due to the fact that the plasmid extractions made on the 3/08/2016 were eluted with water which is not working with this plasmid extraction kit. | ||
| + | |||
| + | |||
| + | [[File:T--Paris_Saclay--160808_gel.JPG|400px|thumb|right|Result of the PCR]] | ||
==== Liquid cultures of bacteria containing plasmid to extract ==== | ==== Liquid cultures of bacteria containing plasmid to extract ==== | ||
| Line 17: | Line 20: | ||
''By Terrence, Alice, Laetitia'' | ''By Terrence, Alice, Laetitia'' | ||
| − | Plasmid sent to sequencing were migrated again on a gel in order to check their size. | + | Plasmid sent to sequencing were migrated again on a gel in order to check their size. A lot of depositions have no DNA which would be explained by the use of water instead of the elution buffer of the plasmid extraction kit. |
[[File:T--Paris_Saclay--160728_visualization_migration_extraction.jpeg|600px|thumb|right|Migration of pPS16_001 (Gblock 1.1), pPS16_002 (Gblock 1.2), pPS16_005, pPS16_006, pUC 19]] | [[File:T--Paris_Saclay--160728_visualization_migration_extraction.jpeg|600px|thumb|right|Migration of pPS16_001 (Gblock 1.1), pPS16_002 (Gblock 1.2), pPS16_005, pPS16_006, pUC 19]] | ||
Latest revision as of 16:24, 9 October 2016
