Difference between revisions of "Team:Paris Saclay/Notebook/August/23"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Tuesday 23<sup>th</sup> August= | = Tuesday 23<sup>th</sup> August= | ||
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===Visualization=== | ===Visualization=== | ||
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| + | ====Colony PCR==== | ||
| + | ''By Léa and Naiane'' | ||
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| + | 12 colonies containing the gBlock GFP 1-9 and 12 colonies transformed with Gibson Assembly products (fragment 3-4) were screened and used for the usual [[Team:Paris_Saclay/Experiments##Polymerase_chain_reaction|protocol]] of Colony PCR. The same colonies were also plated on Petri dish containing solid LB + Cm and liquid culture (LB + Cm) was made from these same colonies. Both petri dishes and the liquid cultures were overnight at 37°C. | ||
| + | The migration on agarose gel was done the next day. | ||
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====Cell Cultures==== | ====Cell Cultures==== | ||
''By Léa, Naiane and Mathilde'' | ''By Léa, Naiane and Mathilde'' | ||
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Interlab study plates from the 22/08/2016 were used to inoculate two tubes containg 5mL of liquid LB medium (chloramphenicol) per construction. | Interlab study plates from the 22/08/2016 were used to inoculate two tubes containg 5mL of liquid LB medium (chloramphenicol) per construction. | ||
| − | DH5a colonies containing | + | DH5a colonies containing pUC19 vector cloned with sgRNA NM (clone 5 and 12) or 1.2 (clones 8 and 14) were inoculated into 4mL of liquid LB medium. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 16:58, 9 October 2016
