Difference between revisions of "Team:Paris Saclay/Notebook/August/29"
MahnaziGEM (Talk | contribs) (→PCR of the plasmid extraction from clonies 4,5,9,12 containing FRB sequence in pJET plasmid) |
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| − | + | {{Team:Paris_Saclay/notebook_header}} | |
| + | = Monday 29<sup>th</sup> August= | ||
| + | ===Visualization=== | ||
====PCR of the plasmid extraction from clonies 4,5,9,12 containing FRB sequence in pJET plasmid==== | ====PCR of the plasmid extraction from clonies 4,5,9,12 containing FRB sequence in pJET plasmid==== | ||
''By Mahnaz'' | ''By Mahnaz'' | ||
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| − | + | [[Team:Paris_Saclay/Experiments#primers|Primers]] used were: | |
{| class="wikitable" | {| class="wikitable" | ||
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|- | |- | ||
|Tm | |Tm | ||
| − | | | + | |60°C |
|- | |- | ||
|t | |t | ||
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| − | [[File:T-- | + | [[File:T--Paris_saclay--2908.extension.png|400px|thumb|center|Result of migration ]] |
| + | The expected size was 474 pb and the 4 plasmids were sent to sequencing. | ||
| + | |||
| + | ====Samples preparation for sequencing==== | ||
| + | ''by Mahnaz'' | ||
| + | |||
| + | 15 µL of plasmides plasmids coding FRB from colonies 4,5,9 and 12 were sent to be sequenced. 20 µL of the primers pJET F (5µM) and pJET R (5µM) were sent for sequencing. | ||
====NanoDrop Measurements==== | ====NanoDrop Measurements==== | ||
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|} | |} | ||
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| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 17:00, 9 October 2016
