Difference between revisions of "Team:Paris Saclay/Notebook/August/29"
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= Monday 29<sup>th</sup> August= | = Monday 29<sup>th</sup> August= | ||
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===Visualization=== | ===Visualization=== | ||
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====PCR of the plasmid extraction from clonies 4,5,9,12 containing FRB sequence in pJET plasmid==== | ====PCR of the plasmid extraction from clonies 4,5,9,12 containing FRB sequence in pJET plasmid==== | ||
''By Mahnaz'' | ''By Mahnaz'' | ||
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| − | + | [[Team:Paris_Saclay/Experiments#primers|Primers]] used were: | |
{| class="wikitable" | {| class="wikitable" | ||
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| − | + | [[File:T--Paris_saclay--2908.extension.png|400px|thumb|center|Result of migration ]] | |
| − | + | The expected size was 474 pb and the 4 plasmids were sent to sequencing. | |
====Samples preparation for sequencing==== | ====Samples preparation for sequencing==== | ||
''by Mahnaz'' | ''by Mahnaz'' | ||
| − | 15 µL of plasmides plasmids coding FRB from colonies 4,5, | + | 15 µL of plasmides plasmids coding FRB from colonies 4,5,9 and 12 were sent to be sequenced. 20 µL of the primers pJET F (5µM) and pJET R (5µM) were sent for sequencing. |
====NanoDrop Measurements==== | ====NanoDrop Measurements==== | ||
Latest revision as of 17:00, 9 October 2016
