Difference between revisions of "Team:Paris Saclay/Notebook/August/31"
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Wednesday 31<sup>th</sup> August= | = Wednesday 31<sup>th</sup> August= | ||
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===Visualization=== | ===Visualization=== | ||
| − | ====Colony PCR of 8 clones containing | + | ====Colony PCR of 8 clones containing fragment 1 and 2 (dCas9 Nm GFP 10) in pSB1C3==== |
''Mahnaz'' | ''Mahnaz'' | ||
| − | Transformed cells which had been grown overnight were used for colony PCR. For that purpose, 8 clones containing dCas9 NM - GFP 10 were screened and used for the usual [[Team:Paris_Saclay/Experiments##Polymerase_chain_reaction|protocol]] of Colony PCR. The same colonies were also plated on Petri dish containing solid LB + 30 µg/mL Cm and liquid cultures (LB + 30 µg/mL Cm) were made from these clones. Both petri dishes and liquid cultures were grown at 37°C. | + | Transformed cells on 26th august which had been grown overnight were used for colony PCR. For that purpose, 8 clones containing dCas9 NM - GFP 10 were screened and used for the usual [[Team:Paris_Saclay/Experiments##Polymerase_chain_reaction|protocol]] of Colony PCR. The same colonies were also plated on Petri dish containing solid LB + 30 µg/mL Cm and liquid cultures (LB + 30 µg/mL Cm) were made from these clones. Both petri dishes and liquid cultures were grown at 37°C. |
For each clones contained in 20 μL water, 5.13 μL of the following mix were added : | For each clones contained in 20 μL water, 5.13 μL of the following mix were added : | ||
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| − | + | [[Team:Paris_Saclay/Experiments#primers|Primers]] used were: | |
{| class="wikitable" | {| class="wikitable" | ||
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|3688 | |3688 | ||
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| + | [[File: T--Paris_saclay--3108.extension.png|400px|thumb|center|Result of migration]] | ||
| + | The result shows that Gibson assembly did not work out. | ||
Latest revision as of 17:01, 9 October 2016
