Difference between revisions of "Team:Paris Saclay/Notebook/September/26"
(→Colony PCR of 16 clones containing FKBP - GFP 10 in pSB1C3 (pPS16_018)) |
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= Monday 26<sup>th</sup> September= | = Monday 26<sup>th</sup> September= | ||
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===Visualization=== | ===Visualization=== | ||
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| − | + | [[Team:Paris_Saclay/Experiments#primers|Primers]] used were: | |
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| − | [[File:T--Paris Saclay-- | + | [[File:T--Paris Saclay--Gel1111111.png|400px|thumb|center|Result of the migration]] |
Two PCR products were at the good size: clones 3 & 11 were selected for sequencing. | Two PCR products were at the good size: clones 3 & 11 were selected for sequencing. | ||
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====Samples preparation for sequencing==== | ====Samples preparation for sequencing==== | ||
| − | + | 'By Maxence, Mahnaz & Caroline' | |
20 µL of plasmids dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) (clones 2, 7 and 8) were sent to be sequenced. 20 µL of the primers iPS168 (5µM) and iPS169 (5µM) were sent for sequencing. | 20 µL of plasmids dCas9 ST - GFP 11 in pSB1C3 (pPS16_017) (clones 2, 7 and 8) were sent to be sequenced. 20 µL of the primers iPS168 (5µM) and iPS169 (5µM) were sent for sequencing. | ||
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4 µL of extracted plasmids and 4 µL of DNA ladder were placed in wells and migrated at 100V during 30 min, in odrder to verify the DNA quantity. | 4 µL of extracted plasmids and 4 µL of DNA ladder were placed in wells and migrated at 100V during 30 min, in odrder to verify the DNA quantity. | ||
| − | + | [[File:T--Paris Saclay--Gel1111112.png|400px|thumb|center|Result of the migration]] | |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 17:09, 9 October 2016
