Difference between revisions of "Team:Dalhousie Halifax NS/Experiments"

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       <h4> DNA Extraction </h4>
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       <h4> 16S Colony PCR </h4>
 
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     <p>Explanation of DNA Extraction here<p>
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     <p>Colony PCR has many applications including; verifying that an insert has been successfully incorporated in plasmid constructs, insert orientation, and species determination of unknown samples. The later was the reason for our use of Colony PCR. After streaking fecal sample onto cellulose plates, we picked colonies of different morphologies to find out which species or genus they were. We did this by using primers that targeted conserved sequence found in bacteria so we could amplify the DNA of almost all bacteria. </p>
     <p><a href="#">MoBio DNA Extraction Protocol</a></p>
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     <p><a href="#">16S Colony PCR Protocol</a></p>
 
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       <h4> DNA Extraction </h4>
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       <h4> ExoSapIt and DNA Cleanup </h4>
 
       <hr id="hr">
 
       <hr id="hr">
 
     </div>
 
     </div>
     <p>Explanation of DNA Extraction here<p>
+
     <p>Instead of PCR clean up, we used ExoSap It. ExoSap It allows for an enzymatic clean-up of left over dNTPs and single stranded DNA. It is a very quick procedure as the only activation ExoSap It needs is a 15 minute 37°C incubation and a 15 minute 80 °C inactivation.</p>
     <p><a href="#">MoBio DNA Extraction Protocol</a></p>
+
     <p><a href="#">ExoSapIt Protocol</a></p>
 
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Revision as of 11:34, 10 October 2016

Dalhousie iGEM 2016

Experiments


High Through-put Sequencing



DNA Extraction


Explanation of DNA Extraction here

MoBio DNA Extraction Protocol


DNA Extraction


Explanation of DNA Extraction here

MoBio DNA Extraction Protocol


DNA Extraction


Explanation of DNA Extraction here

MoBio DNA Extraction Protocol


Isolation of Bacteria



Preparation of Cellulose Media


Congo Red Cellulose media can be used to isolate bacteria that degrade cellulose. It does this because the only carbon source in the media is cellulose. It uses Whatman Paper and gelatin.

Cellulose Media Protocol


16S Colony PCR


Colony PCR has many applications including; verifying that an insert has been successfully incorporated in plasmid constructs, insert orientation, and species determination of unknown samples. The later was the reason for our use of Colony PCR. After streaking fecal sample onto cellulose plates, we picked colonies of different morphologies to find out which species or genus they were. We did this by using primers that targeted conserved sequence found in bacteria so we could amplify the DNA of almost all bacteria.

16S Colony PCR Protocol


ExoSapIt and DNA Cleanup


Instead of PCR clean up, we used ExoSap It. ExoSap It allows for an enzymatic clean-up of left over dNTPs and single stranded DNA. It is a very quick procedure as the only activation ExoSap It needs is a 15 minute 37°C incubation and a 15 minute 80 °C inactivation.

ExoSapIt Protocol