Difference between revisions of "Team:TU-Eindhoven"

 
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<p>Hallo<p>
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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Latest revision as of 16:09, 10 October 2016

iGEM TU Eindhoven

iGEM TU Eindhoven
Small molecule mediated scaffolds
Welcome to the WIKI of the 2016 iGEM team from Eindhoven University of Technology!
  • Read more
    T14-3-3 based scaffold proteins

    In the emerging field of synthetic biology, many new innovations arise. To use them as efficient and safe as possible, regulation is key. Therefore, iGEM TU Eindhoven is developing new kinds of scaffold proteins, based on 14-3-3 proteins. These scaffold proteins have a wide range of applications in nature and can be used to regulate systems in synthetic biology.

  • Read more
    Team

    The iGEM 2016 team of the Eindhoven University of Technology consists of 9 enthusiastic undergraduates of both Biomedical Engineering and Medical Sciences and Technology. Our members work very hard both inside and outside the lab, and have a great time working on our project and learn a lot thanks to iGEM.

  • Read more
    Model

    In order to create new heterodimeric scaffold it is essential to find suitable mutations to create a mutated T14-3-3/CT52 pair that is orthogonal to the wildtype. To find these mutations, The Rosetta software and a self-written protocol was used to determine the yet unknown properties of our newly designed pairs, a model based on Mass-Action and Michaelis-Menten kinetics was developed.

  • Read more
    Human Practices

    Three application scenarios were made to investigate the societal impact our scaffold protein might have. To reach out to community an education package was developed. Furthermore, it was investigated how our project can contribute to safety in synthetic biology.

  • Read more
    Results

    The performance of CT52 fused NanoBiT fragments was analysed by measuring the luminescence at varying concentrations. The data we acquired in the lab by NanoBit assays for our heterodimers was used in order to verify the quality or our mutations. For each newly found mutation set the functionality of the scaffold and the orthogonality with respect to the wildtype were determined. Furthermore a caspase-9 assay was performed to test the activity of caspase-9 after increasing its concentration by T14-3-3.

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