Difference between revisions of "Team:Dalhousie Halifax NS/Results"
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<h3 style="font-family:lato-heavy">This year we managed to isolate a few cellulose degraders and identify them. We also did an extensive proof of concept for our plan to use microbiomes as a "mine" for enzymes and gathered evidence that it would likely work to identify cellulose degrading enzymes. We showed that an E. coli used to degrade soft-wood lumber waste would not be inhibited by the anti-microbial properties of terpenes found in tree's milled for softwood lumber. We also created a couple of new biobricks containing enzymes that we identified bioinformatically from Ruminiclostridium thermocellum, a cellulose degrader identified in the porcupine microbiome. Next year we intend to move forward with the metagenomic library and isolate more enzymes that would be useful for converting soft-wood lumber waste to a better source of fuel (ethanol) or be useful for bioremediation.</h3> | <h3 style="font-family:lato-heavy">This year we managed to isolate a few cellulose degraders and identify them. We also did an extensive proof of concept for our plan to use microbiomes as a "mine" for enzymes and gathered evidence that it would likely work to identify cellulose degrading enzymes. We showed that an E. coli used to degrade soft-wood lumber waste would not be inhibited by the anti-microbial properties of terpenes found in tree's milled for softwood lumber. We also created a couple of new biobricks containing enzymes that we identified bioinformatically from Ruminiclostridium thermocellum, a cellulose degrader identified in the porcupine microbiome. Next year we intend to move forward with the metagenomic library and isolate more enzymes that would be useful for converting soft-wood lumber waste to a better source of fuel (ethanol) or be useful for bioremediation.</h3> | ||
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| + | <p>Below is a much more detailed look at our results starting with what we succeeded in completing and ending with what we intend to work towards next year.</p> | ||
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Revision as of 17:03, 10 October 2016
Results
This year we managed to isolate a few cellulose degraders and identify them. We also did an extensive proof of concept for our plan to use microbiomes as a "mine" for enzymes and gathered evidence that it would likely work to identify cellulose degrading enzymes. We showed that an E. coli used to degrade soft-wood lumber waste would not be inhibited by the anti-microbial properties of terpenes found in tree's milled for softwood lumber. We also created a couple of new biobricks containing enzymes that we identified bioinformatically from Ruminiclostridium thermocellum, a cellulose degrader identified in the porcupine microbiome. Next year we intend to move forward with the metagenomic library and isolate more enzymes that would be useful for converting soft-wood lumber waste to a better source of fuel (ethanol) or be useful for bioremediation.
Below is a much more detailed look at our results starting with what we succeeded in completing and ending with what we intend to work towards next year.
What Worked Out
Chemical Analysis, Proof of Concept and Biobrick
For the Future
What we have planned for the future
