Difference between revisions of "Team:Pasteur Paris/Description"

 
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<h2 id="Trap device">Titre</h2>
 
  
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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
 
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
  
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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<h1><B>Parts description</B></h1>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h3>BBa_K2053000  (for Bronze medal): <i>Thalassiosira pseudonana</i> silaffin kinase (tpSTK1)</h3>
  
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<i>T. pseudonana</i> silaffin kinase (tpSTK1) is a central BioBrick part. It was described as an efficient diatom silaffin-phosphorylating enzyme [1]. Since it was shown that phosphorylation of silaffins increased their biosilification activity [2], we hypothesized that phosphorylation of silica-binding peptide from our fusion protein would increase its biosilification properties.</br></br>
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To facilitate protein purification, we added a HisTag. To remove the HisTag, we separated the HisTag from the sequence by the TEV cleavage site sequence. </br>
  
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<h4>BBa_K2053002 (for Silver medal) : Fusion Si4-cellulose-binding domain-B of domain protein A</h4>
  
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<h5>References</h5>
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In order to functionalize a cellulose-based patch into a biodetection device we designed and created a fusion protein by assembling three parts: the phage displayed silica-binding peptide (Si4) [3],  the <i>Clostridium cellulovorans</i> cellulose-binding domain of cellulose-binding protein A (CBPa) [4], and the B domain of <i>Staphylococcus aureus</i> protein A (BpA) [5]. This fusion protein is used to bind to cellulose-based patch, increase its rigidity by biosilification, and make it a detection device by fixing the Fc fragment of specific antibodies. We used the preexisting iGEM BioBrick parts that we assembled thanks to commonly used flexible linkers [6]. </br></br>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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To facilitate protein purification, we added a His-Tag composed  of 6 histidines at the N-terminus, or C-terminus. To remove the His-Tag, we inserted a TEV (Tobacco Etch virus) cleavage site downstream from the His-Tag. </br></br>
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<h5> Fusion cellulose-binding domain of CBP-B domain of protein A (for Gold medal)</h5>
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We improved previously existing BBa_K863110 and BBa_K103003 BioBricks parts by combining them, creating one part. It is a fusion protein between the <i>Clostridium cellulovorans</i> cellulose-binding domain of cellulose-binding protein A (CBPa) with the B domain of <i>Staphylococcus aureus</i> protein A (BpA). This fusion protein can be used to bind antibodies to cellulose.</br></br>
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To facilitate protein purification, we added a His-Tag. To remove the His-Tag, we separated the HisTag from the sequence by the TEV cleavage site sequence.</br>
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<h2>References: </h2>
  
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[1] Characterization of an Endoplasmic Reticulum-associated Silaffin Kinase from the diatom <i>Thalassiosira pseudonana</i>, Sheppard V et al, 2010, The Journal of Biological Chemistry</br>
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<h5>Inspiration</h5>
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[2] The Role of Proteins in Biosilification, Otzen D, 2012, Scientifica</br>
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<p>See how other teams have described and presented their projects: </p>
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[3] Silica-Precipitating Peptides Isolated from a Combinatorial Phage Display Peptide Library Naik et al, 2002, Journal of Nanoscience and Nanotechnology</br>
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[4] Characterization of the cellulose-binding domain of the <i>Clostridium cellulovorans</i> cellulose-binding protein A, Goldstein MA, 1993, J Bacteriol</br>
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[5] Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from <i>Staphylococcus aureus</i> at 2.9- and 2.8-A resolution, Deisenhofer J, 1981, Biochemistry</br>
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[6] Fusion Protein Linkers: Property, Design and Functionality, Xiaoying C, 2013, Advanced Drug Delivery Reviews</br>
  
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Latest revision as of 09:34, 14 October 2016