Difference between revisions of "Team:SDU-Denmark/Parts"
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<p><strong><a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">BBa_K1763009</a></strong></p> | <p><strong><a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">BBa_K1763009</a></strong></p> | ||
<p><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">This BioBrick we got from </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB sequence core</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"> and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part </span></strong></span></strong><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">, </span></strong></span></strong><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> </span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">and a </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">. </span></strong></span></strong></p> | <p><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">This BioBrick we got from </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp2 AB sequence core</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"> and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part </span></strong></span></strong><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763003" target="_blank">K1763003</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">, </span></strong></span></strong><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a>, <a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a> </span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">and a </span></strong></span></strong><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA/Notebook/Spider_Silk_Genetics/ICA_Oligo_Sequences" target="_blank">initiator</a></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">. </span></strong></span></strong></p> | ||
| + | |||
| + | <p><strong><a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">BBa_K1763010</a> </strong></p> | ||
| + | <p><span style="font-weight: 400;">This BioBrick we got from </span><a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a><span style="font-weight: 400;">. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called </span><a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 AB</a> <span style="font-weight: 400;">and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part </span><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a><span style="font-weight: 400;">, </span><a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a><span style="font-weight: 400;"> and </span><a href="http://parts.igem.org/Part:BBa_K1763004" target="_blank">K1763004</a><span style="font-weight: 400;">.</span></p> | ||
| + | <p><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><strong><br /></strong></strong></span></strong></span></strong><strong><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">BBa_K1763011</a> </strong><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><strong><br /></strong></strong></span></strong></span></strong></p> | ||
| + | <p><span style="font-weight: 400;">This BioBrick we got from </span><a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a><span style="font-weight: 400;">. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called </span><a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 BC</a> <span style="font-weight: 400;">and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part </span><a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a><span style="font-weight: 400;"> and </span><a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">K1763012</a><span style="font-weight: 400;">.</span></p> | ||
| + | <p> </p> | ||
| + | <p><strong><a href="http://parts.igem.org/Part:BBa_K1763012" target="_blank">BBa_K1763012</a></strong> </p> | ||
| + | <p><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">This BioBrick we got from </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA" target="_blank">University of California iGEM 2015 team</a></span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="https://2015.igem.org/Team:UCLA/Project/Programming_Spider_Silk" target="_blank">MaSp1 CA</a> </span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763011" target="_blank">K1763011</a></span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">, </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763010" target="_blank">K1763010</a></span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">, </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763002" target="_blank">K1763002</a></span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"> and </span></strong></span></strong></span></strong><span style="font-weight: 400;"><span style="font-weight: 400;"><a href="http://parts.igem.org/Part:BBa_K1763009" target="_blank">K1763009</a></span></span><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;"><strong><span style="font-weight: 400;">.</span></strong></span></strong></span></strong></p> | ||
</div> | </div> | ||
Revision as of 11:47, 14 October 2016
Parts
This BioBrick contains the coding region of Phasin, a protein derived from R. eutropha. Phasin binds to and reduces the size of PHA or PHB granules????
BioBrick encodes a short hemolysin tag. This tag can be attached to other proteins for secretion through the hemolysin pathway.
This BioBrick contains the native RBS for hemolysin B in R. eutropha. The brick also contains some native DNA prior to the RBS.
This BioBrick contains the coding region of hemolysin B. Hemolysin B is an ABC transporter, that secretes the toxin hemolysin.
This BioBrick contains the native RBS for hemolysin D in R. eutropha. The brick also contains some native DNA prior to the RBS.
This BioBrick contains the coding region for hemolysin D, part of the inner membrane complex of the hemolysin type II secretion pathway.
This BioBrick contains the sequence for the MaSp1 CD gene. This part is a monomer coding for the dragline spider silk MaSp1 and is used for the technique called ICA. This specific part contains specifics overhangs which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.
This BioBrick contains the sequence for the MaSp2 CD gene. This part is a monomer coding for the dragline spider silk MaSp2 and is used for the technique called ICA. This specific part contains specifics overhangs which have sticky ends compatible with e.g. part K2018045, K2018046 and K2018047.
This part is a functional BioBrick contain the coding region of Laterosporulin, with promoter, RBS and terminator. Laterosporulin is a bacteriocin produced by
This BioBrick contain the coding region of,ThuricinS, a bacteriocin produced by Bacillus thuringiensis. ThuricinS target a broad spectrum of pathogens, including Pseudonoas aeruginosa and Enterobactoer Cloacae which is often found in correlation to burn and wound infection.
This BioBrick contain the coding region of LacticinQ, a bacteriocin produced by Lactococcus lactis QU5 and has shown bactericidal activity against Staphylococcus aureus ((Ma et al., 2012)). It function by forming large toroidal pores by distributing membrane lipid organization.
This BioBrick contain the coding region of Laterosporulin-ThuricinS, which is a hybrid bacteriocin with Laterosporulin and ThuricinS we had designed.
This BioBrick contain the coding region of LacticinQ-LacticinZ, which is a hybrid bacteriocin with LacticinQ and LacticinZ we had designed.
>This BioBrick contain the coding region of PyocinS5, a bacteriocin produced by a specific strain of P. aeruginosa and elicit its effect against other strains of P. aeruginosa((Ling et al., 2010)).
This BioBrick contains the coding region of our biofused phasin with a hemolysin A tag, so it is recognized for secretion by the type II hemolysin secretion pathway. This biobrick is designed to work with hemolysin B (K2018027) and hemolysin D (K2018029). This BioBrick will simply bid to PHA granules in the cytoplasm and reduce the size of these.
This part secretes PHB from PHB producing cells.
This part is phaCAB with a hybrid promoter. It consists of K880005 and K934001 .
This part is phaCAB with a hybrid promoter. It consists of K081005 and k934001.
This part is phaCAB with a hybrid promoter. It consists of K608004 and k934001.
This part is phaCAB with a hybrid promoter. It consists of K608003 and K934001.
This part is phaCAB with a hybrid promoter. It consists of J23104 and K934001.
This part is phaCAB with a hybrid promoter. It consists of J23106 and K934001.
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
This BioBrick we got from the University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein, MaSp2. This part is called MaSp2 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp2. The brick is compatible with an initiator and part K1763003.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763002 , K1763009 and K1763004 .
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. This part is called MaSp2 CA and used in the ICA technique and is the third component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763002 and K1763010.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp2. A unique trade for this gene segment is that it codes for the same gene but is different because it contains a primer site. This part is called MaSp2 AB sequence core and used in the ICA technique and is an alternative first component in assembly of a three monomer gene sequence creating a complete MaSp2 gene. The brick is compatible with part K1763003, K1763004, K1763012 and a initiator.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 AB and used in the ICA technique and is the first component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763012 and K1763004.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 BC and used in the ICA technique and is the second component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763010 and K1763012.
This BioBrick we got from University of California iGEM 2015 team. The brick is a monomer for the dragline spider silk protein MaSp1. This part is called MaSp1 CA and used in the ICA technique and is the thrid component in assembly of a three monomer gene sequence creating a complete MaSp1 gene. The brick is compatible with part K1763011, K1763010, K1763002 and K1763009.
