Difference between revisions of "Team:Paris Saclay/Notebook/October/7"

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===BioBrick K2039000 and K2039001 characterization===
 
===BioBrick K2039000 and K2039001 characterization===
  

Latest revision as of 15:55, 14 October 2016

Friday 7th October

BioBrick K2039000 and K2039001 characterization

Culture of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594

By Maxence

The clones put on solid culture the 6th October were put on liquid cultures (LB + 30 µg/mL Cm ou Amp) grown at 37°C overnight:

  • pPS16_018 (FKBP - GFP 10) clone 6
  • pPS16_019 (FRB - GFP 11) clone 4
  • pPS16_009 (GFP 1.9) clone 1

Furthermore, another strain containing the full GFP was cultures in the same conditions: PhB1040 strain 594 (lac-3350 galk2 galT22 2psL179 pGFP) (Amp resistance).