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| + | float:left; |
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| + | width:94%; |
| + | float:left; |
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| + | width;100%; |
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| <div class="row"> | | <div class="row"> |
| <div class="twelve columns centered text-center"> | | <div class="twelve columns centered text-center"> |
− | <h1>Recovery<span>.</span></h1> | + | <h1>Demonstration<span>.</span></h1> |
| <p class="title1" style="text-align:center"></p> | | <p class="title1" style="text-align:center"></p> |
| </div> | | </div> |
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| </div> | | </div> |
| </div> | | </div> |
| + | |
| + | |
| + | <br/><br/><br/> |
| + | |
| + | <h3 class="classic-title"> We summarized the advantages of the Uranium Reaper system in Table 1. </h3> |
| + | <hr style="border:2px dashed; height:2px" color="#666666"> |
| + | <img class="formula" src="" alt=""/> |
| + | <p>Even though the efficiency of the Uranium Reaper system may be somewhat lower than current methods, it can certainly be optimized through further development work. Importantly, Uranium Reaper is much better in other aspects. In the future, we plan to optimize the entire Uranium Reaper strategy in order to enhance the adsorption efficiency.</p> |
| + | |
| | | |
| | | |
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| | | |
| | | |
− | <a id="Design"></a>
| + | <a id="Beyond"></a> |
− | <div class="texttitle anchor" >Design</div> | + | <div class="texttitle" >Achievement beyond experimental work</div> |
| | | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">1.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We submitted about 70 high-quality and well-characterized Standard BioBricks, including a set of derivatives of triple SpyTag and triple SpyCatcher, such as the triple SpyTag-SUP and triple SpyTag-mSA. <a href="https://2016.igem.org/Team:Peking/Basic_Part"/>(Learn more)</a></p> |
| + | </div> |
| + | </div> |
| | | |
− | <br/> | + | <div class="coll"> |
− | <br/> | + | <div class="info"> |
| + | <div class="ordi">2.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We developed a special software which can be used to calculate the molecular weight distribution of protein polymers using Flory’s theory. The results of testing have demonstrated that the software is accurate and useful. <a href="https://2016.igem.org/Team:Peking/Software"/>(Learn more)</a> </p> |
| + | </div> |
| + | </div> |
| | | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">3.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We visited experts from the Peking University departments for Nuclear & Radiochemistry and Physics, respectively, to learn about the current situation surrounding uranium pollution in the real world and how people can control the situation. After finishing the main work, we presented them with the achievements of the project and got their feedback. <a href="https://2016.igem.org/Team:Peking/HP/consulting"/>(Learn more)</a> </p> |
| + | </div> |
| + | </div> |
| | | |
− |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">4.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We did an interview with the Hunan Nuclear Geology 311 Brigade and gained thorough insights into the treatment of uranyl pollution used by the people on the firing line. This way we could compare the methods they were using with the Uranium Reaper strategy. <a href="https://2016.igem.org/Team:Peking/HP/Gold/311"/>(Learn more)</a> </p> |
| + | </div> |
| + | </div> |
| | | |
− | <a id="references"></a> | + | <div class="coll"> |
− | <div class="references">
| + | <div class="info"> |
− | <h3>References:</h3> | + | <div class="ordi">5.</div> |
− | <p>[1] Christopher, D. et al. Streptavidin–biotin technology: improvements and innovations in chemical and biological applications. Appl Microbiol Biotechnol 97, 9343–9353 (2013).</p> | + | </div> |
− | <p>[2] Sau-Ching, W. et al. Engineering monomeric streptavidin and its ligands with infinite affinity in binding but reversibility in interaction. Proteins 77, 404-412 (2009).</p>
| + | <div class="content"> |
| + | <p>We helped and collaborated with 7 other iGEM teams by guiding a new team (BHU-China), as well as discussing about project design and technical skills and sharing DNA materials (OUC-China, BIT-China, Tianjin, UCAS, Jinlin-China and BNU-China). <a href="https://2016.igem.org/Team:Peking/Collaborations"/>(Learn more)</a> </p> |
| + | </div> |
| </div> | | </div> |
| | | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">6.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We attended the CCiC (Central China iGEM Consortium), which is a large-scale competition-free jamboree of about 50 teams, providing participants with an opportunity for meaningful exchanges of ideas and problem solving. <a href="https://2016.igem.org/Team:Peking/Collaborations"/>(Learn more)</a> </p> |
| + | </div> |
| + | </div> |
| + | |
| | | |
| | | |
| + | |
| + | |
| + | <a id="Future"></a> |
| + | <div class="texttitle" >Our future plan</div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">1.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We should reproduce all of the experiments that we have done this summer to make sure the results are credible.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">2.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We will optimize the whole strategy to enhance the adsorption efficiency by changing pH, temperature, reaction time of crosslinking and recovery. (The efficiency is only about 60% without further optimization)</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">3.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>According to the results for the adsorption of 13nM uranyl, the hydrogel exhibited a good ability in a simulated seawater environment. We can thus also look into other usage scenarios of Uranium Reaper, such as bio-mining and uranium enrichment.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">4.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>Exchange of the SUP module for other functional proteins. For example, we can integrate proteins which can bind other heavy metals such as mercury so that the hydrogel can be used to treat other kinds of pollution as well.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">5.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>We can assemble enzyme systems behind the SpyTag backbone to create a production plant in vitro. In the protein hydrogel, the concentration of enzymes can be increased and the efficiency of biocatalysis may consequently also be enhanced.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | <div class="coll"> |
| + | <div class="info"> |
| + | <div class="ordi">6.</div> |
| + | </div> |
| + | <div class="content"> |
| + | <p>If we optimize the number of SpyTag or SpyCatcher modules per protein monomer, as well as the working concentrations of proteins, we may make protein-3D printing using the Spy Crosslinking Network come true.</p> |
| + | </div> |
| + | </div> |
| + | |
| + | |
| + | |
| </div><!--9 columns end--> | | </div><!--9 columns end--> |
| | | |
| </div> | | </div> |
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| + | |
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| </section> | | </section> |