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| | + | <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/Recovery" >Recovery</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li> |
| | + | <li><a href="https://2016.igem.org/Team:Peking/Proof" >Proof and speculation</a></li> |
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| | + | <li><a href="https://2016.igem.org/Team:Peking/HP/otherHP" >Other work</a></li> |
| | + | </ul> |
| | + | </li> |
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| | + | <li><a class="" href="https://2016.igem.org/Team:Peking/Notebook" >Notebook</a></li> |
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| | + | <div id="page-title"> |
| | + | <div class="row"> |
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| | + | <h1>Safety<span>.</span></h1> |
| | + | <p class="title1" style="text-align:center"></p> |
| | + | </div> |
| | + | </div> |
| | + | </div><!-- Page Title End--> |
| | + | |
| | + | |
| | + | <div id="page-content" class="row page"> |
| | + | <div id="primary" class="twelve columns"> |
| | + | <section> |
| | + | <div class="row"> |
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| | + | |
| | + | <div class="three columns"> |
| | + | <div id="page-wrap"> |
| | + | <div id="sidebar" style="color:#000000"> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('overview')">Overview</a></h4> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('design')">Safe Project Design</a></h4> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('lab')">Safe Lab Work</a></h4> |
| | + | <h4><a href="javascript:void(0);" onclick="naver('safe')">Safe Shipment</a></h4> |
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| | + | |
| | | | |
| − | <div id="page-content" class="row page"> | + | |
| − | <div id=" primary" class=" twelve columns"> | + | |
| − | <section>
| + | <div class="nine columns"> |
| − | <div class="row">
| + | |
| | + | <a id="Overview"></a> |
| | + | <div class="texttitle">Overview</div> |
| | + | <br/> |
| | + | <p>This year’s Peking iGEM Team is trying to develop a highly efficient, low-cost and modularized biomaterial to deal with uranium pollution in the environment, using the methods of synthetic biology. Here we wish to share our experiences with safety control.</p> |
| | + | |
| | + | <a id="Overview"></a> |
| | + | <div class="texttitle">Overview</div> |
| | + | <br/> |
| | + | <p>The hydrogel produced in this project is protein network secreted by genetically modified strains of host bacteria. The strains are restricted to the lab so that the cells produce the Uranium Reaper system continuously under rigorous control without any possibility for them to get into contact with the outside environment. Therefore, we have prevented recombinant DNA from flowing out into the environment. Even if a small fraction of bacteria leaked out, they would not express any protein without the induction with IPTG. Additionally, the bacterial hosts are laboratory strains which are likely to be highly uncompetitive compared wild bacteria in the outside environment.</p> |
| | + | <p>During the utilization of the bio-functional hydrogel, uranyl ions are enriched to a high concentration, which is a potential cause for concern. However, the resulting high concentrations of ions are collected along with the bio-functional hydrogel for further processing. In this way, both the uranium and proteins are disposed of appropriately and do not remain in the outside environment.</p> |
| | + | <h3>The details are as follows:</h3> |
| | + | |
| | + | |
| | + | |
| | + | <a id="Beyond"></a> |
| | | | |
| | + | <div class="texttitle">Beyond Experiment</div> |
| | + | <br/> |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">1.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We submitted about 70 high-quality and well-characterized Standard BioBricks, including a set of derivatives of triple SpyTag and triple SpyCatcher, such as the triple SpyTag-SUP and triple SpyTag-mSA. <a href="https://2016.igem.org/Team:Peking/Basic_Part"/>(Learn more)</a></p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">2.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We developed a special software which can be used to calculate the molecular weight distribution of protein polymers using Flory’s theory. The results of testing have demonstrated that the software is accurate and useful. <a href="https://2016.igem.org/Team:Peking/Software"/>(Learn more)</a> </p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">3.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We visited experts from the Peking University departments for Nuclear & Radiochemistry and Physics, respectively, to learn about the current situation surrounding uranium pollution in the real world and how people can control the situation. After finishing the main work, we presented them with the achievements of the project and got their feedback. <a href="https://2016.igem.org/Team:Peking/HP/consulting"/>(Learn more)</a> </p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">4.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We did an interview with the Hunan Nuclear Geology 311 Brigade and gained thorough insights into the treatment of uranyl pollution used by the people on the firing line. This way we could compare the methods they were using with the Uranium Reaper strategy. <a href="https://2016.igem.org/Team:Peking/HP/Gold/311"/>(Learn more)</a> </p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">5.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We helped and collaborated with 7 other iGEM teams by guiding a new team (BHU-China), as well as discussing about project design and technical skills and sharing DNA materials (OUC-China, BIT-China, Tianjin, UCAS, Jinlin-China and BNU-China). <a href="https://2016.igem.org/Team:Peking/Collaborations"/>(Learn more)</a> </p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | + | <div class="coll"> |
| | + | <div class="info"> |
| | + | <div class="ordi">6.</div> |
| | + | </div> |
| | + | <div class="content"> |
| | + | <p>We attended the CCiC (Central China iGEM Consortium), which is a large-scale competition-free jamboree of about 50 teams, providing participants with an opportunity for meaningful exchanges of ideas and problem solving. <a href="https://2016.igem.org/Team:Peking/Collaborations"/>(Learn more)</a> </p> |
| | + | </div> |
| | + | </div> |
| | + | |
| | | | |
| | + | <p> </p> |
| | + | |
| | | | |
| − | </div><!--row end-->
| + | |
| − | </section>
| + | <a id="Future"></a> |
| − | </div><!--12 columes end-->
| + | <div class="texttitle">Our future plan</div> |
| − | </div><!--page-content end--> | + | <br/> |
| − | | + | <div class="coll"> |
| − | | + | <div class="info"> |
| − | | + | <div class="ordi">1.</div> |
| − | | + | </div> |
| − | | + | <div class="content"> |
| − | | + | <p>We should reproduce all of the experiments that we have done this summer to make sure the results are credible.</p> |
| − | | + | </div> |
| − | | + | </div> |
| − | <!-- footer============================================================================== --> | + | |
| − | <style> | + | <div class="coll"> |
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| + | <div class="ordi">2.</div> |
| − | padding-left: 10px;
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| − | padding-right: 12px;
| + | <div class="content"> |
| − | color: #b4bbbb;
| + | <p>We will optimize the whole strategy to enhance the adsorption efficiency by changing pH, temperature, reaction time of crosslinking and recovery. (The efficiency is only about 60% without further optimization)</p> |
| − | } | + | </div> |
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| + | <p>According to the results for the adsorption of 13nM uranyl, the hydrogel exhibited a good ability in a simulated seawater environment. We can thus also look into other usage scenarios of Uranium Reaper, such as bio-mining and uranium enrichment.</p> |
| − | | + | </div> |
| − | <div class="twelve columns" > | + | </div> |
| − | | + | |
| − | <!--<li>© 2014 Sparrow</li> -->
| + | <div class="coll"> |
| − | <li><a href="2016.igem.org/Team:Peking">Home</a> <a href="mailto:pkuigem2016@126.com">Contact</a></li> | + | <div class="info"> |
| − | <span> ©2016 PEKING IGEM. All Rights Reserved.</span>
| + | <div class="ordi">4.</div> |
| − | <li><a href="http://getbootstrap.com/2.3.2/">Based on Bootstrap</a></li> | + | </div> |
| − | </ul>
| + | <div class="content"> |
| − | </div>
| + | <p>Exchange of the SUP module for other functional proteins. For example, we can integrate proteins which can bind other heavy metals such as mercury so that the hydrogel can be used to treat other kinds of pollution as well.</p> |
| − | <div id="go-top" style="display: block;"><a title="Back to Top" href="#">Go To Top</a></div> | + | </div> |
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| − | <script>window.jQuery || document.write('<script src="https://2016.igem.org/Template:Peking/Javascript/jquery_1_10_2_min?action=raw&ctype=text/javascript"><\/script>')</script>
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| + | <div class="content"> |
| − | | + | <p>We can assemble enzyme systems behind the SpyTag backbone to create a production plant in vitro. In the protein hydrogel, the concentration of enzymes can be increased and the efficiency of biocatalysis may consequently also be enhanced.</p> |
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| − | <!--quotations from black: start--> | + | <div class="ordi">6.</div> |
| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery?action=raw&ctype=text/javascript"></script> | + | </div> |
| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_sticky?action=raw&ctype=text/javascript"></script> | + | <div class="content"> |
| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/jquery_easing_1_3_pack?action=raw&ctype=text/javascript"></script> | + | <p>If we optimize the number of SpyTag or SpyCatcher modules per protein monomer, as well as the working concentrations of proteins, we may make protein-3D printing using the Spy Crosslinking Network come true.</p> |
| − | <script type='text/javascript' src="https://2016.igem.org/Template:Peking/Javascript/bootstrap_min?action=raw&ctype=text/javascript"></script> | + | </div> |
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