Difference between revisions of "Team:Peking/Basic Part"

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                    <li class="menu-1"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking" >Home</a></li>
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                            <li class="menu-1"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking" >Home</a></li>
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                    <li class="dropdown menu-2"><a class="dropdown-toggle" data-toggle="dropdown" href="#" > Achievements</a>
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                            <li><a href="https://2016.igem.org/Team:Peking/Demonstrate" >Results</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Demonstrate" >Results</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Basic_Part" >Parts</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Collaborations" >Collaborations</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Collaborations" >Collaborations</a></li>
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                    <li class="dropdown menu-3"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project</a>
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                            <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Description" >Overview</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Design" >Design</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Crosslinking" >Crosslinking</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption" >Uranyl adsorption</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Clearance" >Clearance</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Clearance" >Clearance</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Secretion" >Secretion</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/Proof" >Final Performance</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Proof" >Final Performance</a></li>
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                    <li class="dropdown menu-4"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Modeling</a>
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                            <li><a href="https://2016.igem.org/Team:Peking/Model" >Protein polymerization</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/Model" >Protein polymerization</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/Gold" >Overview</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/HP/Gold" >Overview</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li>
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                                    <li><a href="https://2016.igem.org/Team:Peking/HP/311" >Field research</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/questionnaire" >Questionnaire</a></li>
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                            <li><a href="https://2016.igem.org/Team:Peking/HP/otherHP" >Other work</a></li>
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                    <li class="menu-6"><a class="colapse-menu1" href="https://2016.igem.org/Team:Peking/Safety" >Safety</a>
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                             <h3 class="classic-title">Basic structure of the following parts</h3>
 
                             <h3 class="classic-title">Basic structure of the following parts</h3>
 
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                             <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/c/c5/T—Peking--image_partfig1.png" alt=""/></p>
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                             <figure>
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                                <img class="featurette-image" src="https://static.igem.org/mediawiki/2016/c/c5/T—Peking--image_partfig1.png" style="width:60%;" alt=""/>
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                             <h3 class="classic-title">BBa_K1989000</h3>
 
                             <h3 class="classic-title">BBa_K1989000</h3>
 
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                            <p style="text-align:center;"><img style="width: 60% ;" src=" https://static.igem.org/mediawiki/2016/e/e9/T--Peking--image_partfig2.png" alt=""/></p>
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                             <p class="lead add-bottom" style="color:#5E5656">Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed. </p>
 
                             <p class="lead add-bottom" style="color:#5E5656">Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed. </p>
 
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                             <h3 class="classic-title">BBa_K1989002</h3>
 
                             <h3 class="classic-title">BBa_K1989002</h3>
 
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                          <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/d/d9/T--Peking--image_partfig4.png " alt=""/></p>
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                            <p style="text-align:center;"><img style="width: 60% ;" src="https://static.igem.org/mediawiki/2016/d/d9/T--Peking--image_partfig4.png " alt=""/></p>
 
                             <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with SUP (BBa_K1989000) our hydrogel system is constructed.</p>
 
                             <p class="lead add-bottom" style="color:#5E5656">Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with SUP (BBa_K1989000) our hydrogel system is constructed.</p>
 
                              
 
                              
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                                 <div id='groupparts' style='min-height:100px;width:700px;'><div style='width:300px;margin:2px;padding:20px;color:gray;border:1px solid gray'>Loading…..</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM2016', g:'Peking' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
 
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                             <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.</p>
 
                             <p class="lead add-bottom" style="color:#5E5656">Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.</p>
 
                              
 
                              
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Revision as of 17:19, 16 October 2016

Parts

Parts

Favorite Parts

Basic structure of the following parts


BBa_K1989000

Super uranyl-binding protein (SUP) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the uranyl-binding capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed.


BBa_K1989001

Triple spycatcher was spaced by 15*(VPGVG). Paired with triple spytag (BBa_K1989000) and tripled spytag with mSA (BBa_K1989002) our hydrogel system is constructed.

BBa_K1989002

Monomeric streptavidin (mSA) was fused to 4*(VPGVG) spaced triple spytag. The fused protein is used to generate the recyclable capacity in our hydrogel system. Paired with triple spycatcher (BBa_K1989001) and tripled spytag with SUP (BBa_K1989000) our hydrogel system is constructed.


Part Table

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Part Collection

2016 Peking iGEM team members devoted themselves to constructing a part library of the multifunctional material. We have not only submitted all of new basic Spy network parts, but also provided multiple composite parts combining Spy network with other metal binding proteins and fluorescent proteins. We also constructed a library of signal peptides and validated the secretion capacity of our material.

Functions of all the parts were tested and validated both in controlled laboratory condition and in normal condition. The parts allow simple modules insertion using 3A Assembly, Gibson and Golden Gate Assembly which making them convenient and powerful tools for other iGEM team.