Difference between revisions of "Team:Virginia/Parts"
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This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence. | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence. | ||
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<span><img style="height:310px; width: 272px; float:right" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png"/> | <span><img style="height:310px; width: 272px; float:right" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png"/> | ||
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Revision as of 17:56, 16 October 2016
Overview
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
