Line 46: | Line 46: | ||
<br><br><span class="ptitle">Bba_K1879001</span><br><br> | <br><br><span class="ptitle">Bba_K1879001</span><br><br> | ||
− | + | This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | |
− | + | ||
Revision as of 19:52, 16 October 2016
![](https://static.igem.org/mediawiki/2016/9/93/T--Virginia--Parts_header.png)
Overview
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
![](https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png)
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) cleavage enzyme necessary to remove the CBZ protecting group from the N-terminus of amino acids. The coding sequence was derived from Nanduri et al (Ref), and we included an RBS sequence upstream of the coding sequence.
![](https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png)
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.