Difference between revisions of "Team:Newcastle/Proof/MFC"

Line 9: Line 9:
  
 
<i>Microbial Fuel Cell with E. coli and Arabinose</i>
 
<i>Microbial Fuel Cell with E. coli and Arabinose</i>
−
<p>After discussing the ethical issues of using yeast with <a href="https://2016.igem.org/Team:Newcastle/Integrated_Practices">PEALS</a>  
+
<p>After discussing the ethical issues of using yeast with <a href="https://2016.igem.org/Team:Newcastle/Integrated_Practices">PEALS</a>, we decided to change our constructs to <i>E. coli</i>. However, we generally used the same protocol as above and made only slight changes, such as using 1g of arabinose as well as the 9g of glucose dissolved in 50ml of potassium phosphate buffer. Around ??ml of the BBa_K1895100 and the BBa_K1895101 was added to individual fuel cells. </p>
  
−
Using the same protocol as above
+
<p>The fuel cell was left to run for an hour and the voltage was taken every 3 minutes.<p>
  
  

Revision as of 20:23, 16 October 2016



Microbial Fuel Cell with Yeast

Our original experiment, which looked at making a microbial fuel cell using yeast, followed the University of Reading’s protocol kindly given to us by Dr Ed Milner, Dr Paniz Izadi and Professor Ian Head. The protocol can be seen here

Microbial Fuel Cell with E. coli and Arabinose

After discussing the ethical issues of using yeast with PEALS, we decided to change our constructs to E. coli. However, we generally used the same protocol as above and made only slight changes, such as using 1g of arabinose as well as the 9g of glucose dissolved in 50ml of potassium phosphate buffer. Around ??ml of the BBa_K1895100 and the BBa_K1895101 was added to individual fuel cells.

The fuel cell was left to run for an hour and the voltage was taken every 3 minutes.

1g of arabinose, 9g of glucose dissolved in 50ml of Potassium Phosphate Buffer.