Difference between revisions of "Team:Virginia/Parts"
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<p><span class="p"> | <p><span class="p"> | ||
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) <br> | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) <br> | ||
| − | cleavage enzyme necessary to remove the CBZ protecting group from the | + | cleavage enzyme necessary to remove the CBZ protecting group from the <br> |
| − | + | N-terminus of amino acids. The coding sequence was derived from Nanduri <br> | |
| − | an RBS sequence upstream of the coding sequence.</span></p> | + | et al (Ref), and we included an RBS sequence upstream of the coding sequence.</span></p> |
<br><br><br><br><br><br><br><br><br><br><br><br> | <br><br><br><br><br><br><br><br><br><br><br><br> | ||
Revision as of 21:25, 16 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al (Ref), and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
