Difference between revisions of "Team:Virginia/Parts"
| Line 48: | Line 48: | ||
<br><br><span class="stitle">Bba_K1879001</span><br><br> | <br><br><span class="stitle">Bba_K1879001</span><br><br> | ||
| − | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ<br> | + | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage<br> |
| − | + | enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment <br> | |
| − | + | system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This<br> | |
| − | + | part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino<br> | |
| − | + | or hydroxyl groups in organic reactions. | |
</span></p> | </span></p> | ||
Revision as of 21:27, 16 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al (Ref), and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment
system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This
part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino
or hydroxyl groups in organic reactions.
