Difference between revisions of "Team:IngenuityLab Canada/Parts"

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<div class="article-cap"><strong>Figure 5:</strong> Schematic of the 3 parts that we will be submitting for the BioBrick requirement. Specifically, the first 2 parts for submission includes the CP47 subunit of Photosystem II fused to a 6x His-tag. BBa_K2127001, is unique due to the addition of TFBS (Transcription Factor Binding Site) that has been shown in literature to act as a super-promoter in native <i>Synechocystis sp.</i> PCC 6803. The last part for submission include the psbT subunit of Photosystem II as a standalone part.  All parts contain a lac promoter, lac operator, RBS, two-stop codons, and a terminator sequence. All parts are synthesized as shown by IDT DNA Inc.</div>
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<div class="article-cap"><strong>Figure 1:</strong> Schematic of the 3 parts that we will be submitting for the BioBrick requirement. Specifically, the first 2 parts for submission includes the CP47 subunit of Photosystem II fused to a 6x His-tag. BBa_K2127001, is unique due to the addition of TFBS (Transcription Factor Binding Site) that has been shown in literature to act as a super-promoter in native <i>Synechocystis sp.</i> PCC 6803. The last part for submission include the psbT subunit of Photosystem II as a standalone part.  All parts contain a lac promoter, lac operator, RBS, two-stop codons, and a terminator sequence. All parts are synthesized as shown by IDT DNA Inc.</div>
 
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Revision as of 02:04, 17 October 2016

Ingenuity Lab - dNANO

 

Parts

Part development:

We plan to submit the following 3 BioBrick parts for this year’s competition.

http://ingenuitylab.ca/igem/images/project_overview_fig5.jpg
Figure 1: Schematic of the 3 parts that we will be submitting for the BioBrick requirement. Specifically, the first 2 parts for submission includes the CP47 subunit of Photosystem II fused to a 6x His-tag. BBa_K2127001, is unique due to the addition of TFBS (Transcription Factor Binding Site) that has been shown in literature to act as a super-promoter in native Synechocystis sp. PCC 6803. The last part for submission include the psbT subunit of Photosystem II as a standalone part. All parts contain a lac promoter, lac operator, RBS, two-stop codons, and a terminator sequence. All parts are synthesized as shown by IDT DNA Inc.