FlashLab parts

The device is composed of a commercial fluidic chip:

The chip is open on the button part and closed with a standard microscope cover glass (0.3[mm] thick).

FlashLab setup

The setup of the device is composed of two parts, as shown below (Figure 3):

FlashLab test

Once the sample is loaded, it diffuses into the channel. If the sample contains a repellent, the bacteria will react and move away from it as shown below:

This will cause changes in the bacteria concentration: very low concentration, where the repellent diffused to, next to a very high concentration, where the bacteria moved to (right picture, figure 1:4). Those changes will also be visible, as the higher concentration of colored bacteria manifests itself in a stronger color (blue gradient, figures 1:3 and 1:4).
If the sample does not contain target material, the bacteria will not react and no gradient will form.
For more information see mathematical model.

FlashLab parts:

The set up for the device is as shown in "Design and Implementation". The bacteria, E.coli strain #$%^&@#$%^, was taken from a petri dish and diluted in 180[µL] of motility buffer. The Chemo-repellent used was 30 [µL] of #$%^&@#$%^ in concentration of α[M] while the control was motility buffer. The pictures were taken in differential of apart.

Following γ[min], a noticeable gradient of blue color formed in the chip's channel. on the far left, a relatively light shade. Next to it, an area with a darker tone and from there up to the right end of the channel, the tone didn’t change at all. This lines up with the theory perfectly, as the lighter shade is caused by colored bacteria moving away from the repellent. The darker shade is the clustering of bacteria in the chemo-repellent diffusion limit. All other bacteria in the channel, were not exposed to the repellent and so didn’t react.

==========For more information see chip experiment protocol==========

FlashLab has advatages as a detection tool: - Cheap The only major cost is the fluidic chip and one costs about 15$ and can be reuse multible times.
- Fast as shown in the expermints, it takes about 30 minutes for detection. This is faster then most other bacterial detection (based on transecription and translation) and most laboratory tests (HPLC).
- Verstile The S.tar system enable this device to detect verity of materials: hormones, amino acids, PCE etc.
- Senstive: Bacteria can sense extrimly small traces of target material.
- Easy to use: The set up of the system is an easy, two parts process. To reuse the chip you only need to flush the channel with water and dry.

======(For Farther improvement see "Design and Devolpment")======