Tuesday 21st June

Lab work

Competent cells preparation

by Lea, Caroline and Marion

Culture OD was measured at 600nm (OD = 3.4). 3.4mL were put into 250mL of LB medium and incubated at 20°C and 180rpm overnight.

Interlab study

Transformation

by Lea, Caroline and Marion

50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. Petri dish were prepared with selective medium containing LB, agar and 30µg/mL chloramphenicol. Each transformation condition was displayed into petri dish in duplicate and then incubated at 37°C overnight.