Monday 4^th July

Lab work

Visualization

Digestion of plasmids

By Mathilde and Alice

The following plasmids were digested again with EcoR1 and HindIII:

• 1.1 (from the 6 clones that were selected on 29/06/16)
• 2.1 (from the 6 clones that were selected on 29/06/16)
• 3.1 (from the 6 clones that were selected on 29/06/16)
• 3.2 (from the 6 clones that were selected on 29/06/16)

The same digestion protocol as on Template:Font color was followed increasing the DNA quantity (10µL DNA, 7µL water, 2µL Red buffer, 0.5µL EcoR1, 0.5µL HindIII). Digestion products were migrated on agarose gel. The DNA concentration was still insufficient meaning the extraction step was not efficient. New extraction will be performed on clones got after the transformation.

Bringing DNA closer

Plasmids extraction

By Naiane and Laetitia

Plasmids from the following strains were extracted with the kit "Charge Switch-Pro Plasmid Miniprep":

• DS-NMCas
• DS-SPCasN
• DS-ST1
• DS-TDCasN

Then plasmids were digested with AvrII increasing the DNA quantity (10µL DNA, 1µL AvrII, 2µL Tango buffer, 7µL water). Digestion products were migrated (5µL digestion, 5µL water, 2µL Template:Font color