Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

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! Buffer Z (250mL)
 
! Buffer Z (250mL)
 
! Buffer Z (250mL)
 
! Buffer Z (250mL)

Revision as of 16:15, 6 July 2016

Tuesday 1st July

Lab Work

Biobrick characterization

Buffer preparations

by Charlene and Caroline

Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL)
Na2HPO4 – 3,125g 
 NaH2PO4.H2O – 1,38g
 KCl – 0,185g
 MgSO4 – 0,04g
 β-mercaptoethanol – 0,893mL
Trisphosphate pH 7,8 1M – 6,25mL 
 MgCl2 2M – 1mL
 DTT 1M -0,25mL
 EDTA 0,5M – 0,5mL
 BSA – 0,25g
 Glycerol 60% - 62,5mL
 Triton 10% (100x) – 25mL
Na2CO3 1M – 0,78g