|
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Line 16: |
Line 16: |
| | | |
| NaH2PO4.H2O – 1,38g | | NaH2PO4.H2O – 1,38g |
| + | |
| KCl – 0,185g | | KCl – 0,185g |
− | MgSO4 – 0,04g
| + | |
− | β-mercaptoethanol – 0,893mL
| + | MgSO4 – 0,04g |
− | | Trisphosphate pH 7,8 1M – 6,25mL | + | |
− | MgCl2 2M – 1mL
| + | β-mercaptoethanol – 0,893mL |
− | DTT 1M -0,25mL
| + | |
− | EDTA 0,5M – 0,5mL
| + | | |
− | BSA – 0,25g
| + | Trisphosphate pH 7,8 1M – 6,25mL |
− | Glycerol 60% - 62,5mL
| + | |
− | Triton 10% (100x) – 25mL
| + | MgCl2 2M – 1mL |
− | | Na2CO3 1M – 0,78g | + | |
| + | DTT 1M -0,25mL |
| + | |
| + | EDTA 0,5M – 0,5mL |
| + | |
| + | BSA – 0,25g |
| + | |
| + | Glycerol 60% - 62,5mL |
| + | |
| + | Triton 10% (100x) – 25mL |
| + | | |
| + | Na2CO3 1M – 0,78g |
| |} | | |} |
Revision as of 16:17, 6 July 2016