Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

Line 16: Line 16:
  
 
NaH2PO4.H2O – 1,38g
 
NaH2PO4.H2O – 1,38g
 +
 
KCl – 0,185g
 
KCl – 0,185g
  MgSO4 – 0,04g
+
 
  β-mercaptoethanol – 0,893mL
+
MgSO4 – 0,04g
| Trisphosphate pH 7,8 1M – 6,25mL
+
 
  MgCl2 2M – 1mL
+
β-mercaptoethanol – 0,893mL
  DTT 1M -0,25mL
+
 
  EDTA 0,5M – 0,5mL
+
|  
  BSA – 0,25g
+
Trisphosphate pH 7,8 1M – 6,25mL
  Glycerol 60% - 62,5mL
+
 
  Triton 10% (100x) – 25mL
+
MgCl2 2M – 1mL
| Na2CO3 1M – 0,78g
+
 
 +
DTT 1M -0,25mL
 +
 
 +
EDTA 0,5M – 0,5mL
 +
 
 +
BSA – 0,25g
 +
 
 +
Glycerol 60% - 62,5mL
 +
 
 +
Triton 10% (100x) – 25mL
 +
|  
 +
Na2CO3 1M – 0,78g
 
|}
 
|}

Revision as of 16:17, 6 July 2016

Tuesday 1st July

Lab Work

Biobrick characterization

Buffer preparations

by Charlene and Caroline

Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL)

Na2HPO4 – 3,125g

NaH2PO4.H2O – 1,38g

KCl – 0,185g

MgSO4 – 0,04g

β-mercaptoethanol – 0,893mL

Trisphosphate pH 7,8 1M – 6,25mL

MgCl2 2M – 1mL

DTT 1M -0,25mL

EDTA 0,5M – 0,5mL

BSA – 0,25g

Glycerol 60% - 62,5mL

Triton 10% (100x) – 25mL

Na2CO3 1M – 0,78g