Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

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Na2CO3 1M – 0,78g
 
Na2CO3 1M – 0,78g
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===Visualization===
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====Migration of plasmids containing gBlocks digestion====
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Products of the digestion made the 30/06/16 are migrated on a gel (0.8% agarose) during 30 min, 100 V.
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{| class="wikitable"
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|-
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! Component
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! Volume (µL)
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|-
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| Digested DNA
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| 10
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|-
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| Loading buffer
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| 2
 
|}
 
|}

Revision as of 16:26, 6 July 2016

Tuesday 1st July

Lab Work

Biobrick characterization

Buffer preparations

by Charlene and Caroline

Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL)

Na2HPO4 – 3,125g

NaH2PO4.H2O – 1,38g

KCl – 0,185g

MgSO4 – 0,04g

β-mercaptoethanol – 0,893mL

Trisphosphate pH 7,8 1M – 6,25mL

MgCl2 2M – 1mL

DTT 1M -0,25mL

EDTA 0,5M – 0,5mL

BSA – 0,25g

Glycerol 60% - 62,5mL

Triton 10% (100x) – 25mL

Na2CO3 1M – 0,78g

Visualization

Migration of plasmids containing gBlocks digestion

Products of the digestion made the 30/06/16 are migrated on a gel (0.8% agarose) during 30 min, 100 V.

Component Volume (µL)
Digested DNA 10
Loading buffer 2