Difference between revisions of "Team:Paris Saclay/Parts"

(→‎Existing parts used for the project)
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{{Team:Paris_Saclay/project_header|titre=Parts}}
 
{{Team:Paris_Saclay/project_header|titre=Parts}}
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<html><style>header{background-image: url("https://static.igem.org/mediawiki/2016/9/97/T--Paris_Saclay--banniere_parts.jpg");}</style></html>
  
 
=Existing parts used for the project=
 
=Existing parts used for the project=
  
−
In order to construct or own parts, we chose several existing parts from the registry to complete the coding sequence qith promoters, RBS and terminators:
+
In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
  
  
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!Length (bp)
 
!Length (bp)
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_J23119/ BBa_J23119]
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|[http://parts.igem.org/Part:BBa_J23119 BBa_J23119]
 
|Basic
 
|Basic
 
|Constitutive promoter family member
 
|Constitutive promoter family member
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|35
 
|35
 
|-
 
|-
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|[http://parts.igem.org/Part:BBa_B0030/ BBa_B0030]
+
|[http://parts.igem.org/Part:BBa_B0030 BBa_B0030]
 
|Basic
 
|Basic
 
|RBS.1 (strong) - modified from R. Weiss
 
|RBS.1 (strong) - modified from R. Weiss
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|15
 
|15
 
|-
 
|-
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|[http://parts.igem.org/Part:BBa_B0015/ BBa_B0015]
+
|[http://parts.igem.org/Part:BBa_B0015 BBa_B0015]
 
|Basic
 
|Basic
 
|Double terminator (B0010-B0012) (rrnB T1 - T7Te)
 
|Double terminator (B0010-B0012) (rrnB T1 - T7Te)
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=Parts designed for the project=
 
=Parts designed for the project=
  
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In order to achieve our project; we designed several basic and composite parts:
+
In order to successfully develop our project, we designed several basic and composite parts:
 +
 
  
 
{| class="wikitable"
 
{| class="wikitable"
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!Length (bp)
 
!Length (bp)
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_K2039003/ BBa_K2039003]
+
|[http://parts.igem.org/Part:BBa_K2039003 BBa_K2039003]
 
|Basic
 
|Basic
 
|Coding sequences of proteins FKBP and GFP10  
 
|Coding sequences of proteins FKBP and GFP10  
 
|528
 
|528
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_K2039004/ BBa_K2039004]
+
|[http://parts.igem.org/Part:BBa_K2039004 BBa_K2039004]
 
|Basic
 
|Basic
 
|Coding sequences of proteins FRB* and GFP11
 
|Coding sequences of proteins FRB* and GFP11
 
|498
 
|498
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_K2039000/ BBa_K2039000]
+
|[http://parts.igem.org/Part:BBa_K2039005 BBa_K2039005]
 +
|Basic
 +
|Coding sequences of protein GFP 1.9
 +
|633
 +
|-
 +
|[http://parts.igem.org/Part:BBa_K2039000 BBa_K2039000]
 
|Composite
 
|Composite
 
|Part expressing the dimerization protein FKBP and GFP10
 
|Part expressing the dimerization protein FKBP and GFP10
 
|731
 
|731
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_K2039001/ BBa_K2039001]
+
|[http://parts.igem.org/Part:BBa_K2039001 BBa_K2039001]
 
|Composite
 
|Composite
 
|Part expressing the dimerization protein FRB* and GFP11
 
|Part expressing the dimerization protein FRB* and GFP11
 
|701
 
|701
 
|-
 
|-
−
|[http://parts.igem.org/Part:BBa_K2039002/ BBa_K2039002]
+
|[http://parts.igem.org/Part:BBa_K2039006 BBa_K2039006]
 
|Composite
 
|Composite
−
|Dimerization system FRB/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
+
|Part expressing the protein GFP 1.9
 +
|836
 +
|-
 +
|[http://parts.igem.org/Part:BBa_K2039002 BBa_K2039002]
 +
|Composite
 +
|Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
 
|1440
 
|1440
 +
|-
 +
|[http://parts.igem.org/Part:BBa_K2039007 BBa_K2039007]
 +
|Composite
 +
|Dimerization system FRB*/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9)
 +
|2284
 
|-
 
|-
 
|}
 
|}
  
 
{{Team:Paris_Saclay/project_footer}}
 
{{Team:Paris_Saclay/project_footer}}

Latest revision as of 10:21, 18 October 2016

Parts

Existing parts used for the project

In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:


Name Type Description Designer Length (bp)
[http://parts.igem.org/Part:BBa_J23119 BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35
[http://parts.igem.org/Part:BBa_B0030 BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15
[http://parts.igem.org/Part:BBa_B0015 BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129

Parts designed for the project

In order to successfully develop our project, we designed several basic and composite parts:


Name Type Description Length (bp)
[http://parts.igem.org/Part:BBa_K2039003 BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528
[http://parts.igem.org/Part:BBa_K2039004 BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498
[http://parts.igem.org/Part:BBa_K2039005 BBa_K2039005] Basic Coding sequences of protein GFP 1.9 633
[http://parts.igem.org/Part:BBa_K2039000 BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731
[http://parts.igem.org/Part:BBa_K2039001 BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701
[http://parts.igem.org/Part:BBa_K2039006 BBa_K2039006] Composite Part expressing the protein GFP 1.9 836
[http://parts.igem.org/Part:BBa_K2039002 BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440
[http://parts.igem.org/Part:BBa_K2039007 BBa_K2039007] Composite Dimerization system FRB*/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2284





Université Paris Sud
91405 Orsay, France
Copyright © 2016 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.