Difference between revisions of "Team:Paris Saclay/Parts"

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{{Team:Paris_Saclay/project_header|titre=Parts}}
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<html><style>header{background-image: url("https://static.igem.org/mediawiki/2016/9/97/T--Paris_Saclay--banniere_parts.jpg");}</style></html>
  
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=Existing parts used for the project=
  
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In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:
  
  
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{| class="wikitable"
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|-
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!Name
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!Type
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!Description
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!Designer
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!Length (bp)
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|-
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|[http://parts.igem.org/Part:BBa_J23119 BBa_J23119]
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|Basic
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|Constitutive promoter family member
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|iGEM2006_Berkeley
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|35
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|-
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|[http://parts.igem.org/Part:BBa_B0030 BBa_B0030]
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|Basic
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|RBS.1 (strong) - modified from R. Weiss
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|Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003
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|15
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|-
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|[http://parts.igem.org/Part:BBa_B0015 BBa_B0015]
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|Basic
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|Double terminator (B0010-B0012) (rrnB T1 - T7Te)
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|Reshma Shetty, 2003
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|129
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|-
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|}
  
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=Parts designed for the project=
  
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<div class="column full_size">
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In order to successfully develop our project, we designed several basic and composite parts:
  
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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{| class="wikitable"
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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|-
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!Name
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!Type
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!Description
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!Length (bp)
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|-
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|[http://parts.igem.org/Part:BBa_K2039003 BBa_K2039003]
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|Basic
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|Coding sequences of proteins FKBP and GFP10
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|528
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|-
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|[http://parts.igem.org/Part:BBa_K2039004 BBa_K2039004]
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|Basic
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|Coding sequences of proteins FRB* and GFP11
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|498
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|-
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|[http://parts.igem.org/Part:BBa_K2039005 BBa_K2039005]
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|Basic
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|Coding sequences of protein GFP 1.9
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|633
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|-
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|[http://parts.igem.org/Part:BBa_K2039000 BBa_K2039000]
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|Composite
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|Part expressing the dimerization protein FKBP and GFP10
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|731
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|-
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|[http://parts.igem.org/Part:BBa_K2039001 BBa_K2039001]
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|Composite
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|Part expressing the dimerization protein FRB* and GFP11
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|701
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|-
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|[http://parts.igem.org/Part:BBa_K2039006 BBa_K2039006]
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|Composite
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|Part expressing the protein GFP 1.9
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|836
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|-
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|[http://parts.igem.org/Part:BBa_K2039002 BBa_K2039002]
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|Composite
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|Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11)
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|1440
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|-
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|[http://parts.igem.org/Part:BBa_K2039007 BBa_K2039007]
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|Composite
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|Dimerization system FRB*/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9)
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|2284
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|-
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|}
  
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{{Team:Paris_Saclay/project_footer}}
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<div class="highlight">
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<groupparts>iGEM2016 Example</groupparts>
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Latest revision as of 10:21, 18 October 2016

Parts

Existing parts used for the project

In order to construct our own parts, we chose several existing parts from the registry to complete the coding sequence with promoters, RBS and terminators:


Name Type Description Designer Length (bp)
[http://parts.igem.org/Part:BBa_J23119 BBa_J23119] Basic Constitutive promoter family member iGEM2006_Berkeley 35
[http://parts.igem.org/Part:BBa_B0030 BBa_B0030] Basic RBS.1 (strong) - modified from R. Weiss Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003 15
[http://parts.igem.org/Part:BBa_B0015 BBa_B0015] Basic Double terminator (B0010-B0012) (rrnB T1 - T7Te) Reshma Shetty, 2003 129

Parts designed for the project

In order to successfully develop our project, we designed several basic and composite parts:


Name Type Description Length (bp)
[http://parts.igem.org/Part:BBa_K2039003 BBa_K2039003] Basic Coding sequences of proteins FKBP and GFP10 528
[http://parts.igem.org/Part:BBa_K2039004 BBa_K2039004] Basic Coding sequences of proteins FRB* and GFP11 498
[http://parts.igem.org/Part:BBa_K2039005 BBa_K2039005] Basic Coding sequences of protein GFP 1.9 633
[http://parts.igem.org/Part:BBa_K2039000 BBa_K2039000] Composite Part expressing the dimerization protein FKBP and GFP10 731
[http://parts.igem.org/Part:BBa_K2039001 BBa_K2039001] Composite Part expressing the dimerization protein FRB* and GFP11 701
[http://parts.igem.org/Part:BBa_K2039006 BBa_K2039006] Composite Part expressing the protein GFP 1.9 836
[http://parts.igem.org/Part:BBa_K2039002 BBa_K2039002] Composite Dimerization system FRB*/FKBP linked to two subunit of tripartite GFP (GFP 10 & GFP 11) 1440
[http://parts.igem.org/Part:BBa_K2039007 BBa_K2039007] Composite Dimerization system FRB*/FKBP linked to tripartite GFP (GFP 10, GFP 11 & GFP 1.9) 2284





Université Paris Sud
91405 Orsay, France
Copyright © 2016 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.