Difference between revisions of "Team:Newcastle/Notebook/Lab/Lightbulb/Week 12"

 
Line 41: Line 41:
 
<body>
 
<body>
 
<div class="column full_size">
 
<div class="column full_size">
 +
 
<h2>September 5th 2016</h2>
 
<h2>September 5th 2016</h2>
−
<p>We planned to mini prep our cell samples but Matt said that the IDT samples had not grown enough so we incubated them for another day. We then designed primers for PCR primer. In the afternoon we mini-prepped the DNA 2.0 using Invitrogen Purelink Quick Plasmid Mini-prep kit.</p>  
+
<p>We planned to mini prep our cell samples but Matt said that the IDT samples had not grown enough so we incubated them for another day. We then designed primers for PCR primer. In the afternoon we mini-prepped the DNA2.0 using a <a href="https://www.thermofisher.com/order/catalog/product/K210010">ThermoFischer Purelink Quick Plasmid Miniprep kit</a>.</p>  
 +
 
 
<h2>September 6th 2016</h2>
 
<h2>September 6th 2016</h2>
−
<p>This morning we min-pepped our cells after they had been left to grow for a further 24 hours. This was so we could send our constructs to iGEM headquarters and get them sequenced. Again we used the Invitrogen Purelink Quick Plasmid Mini-prep kit. We did have 9 samples (using 50uL plate samples) but after a slight mishap, we were left with 8 samples.</p>
+
<p>This morning we mini-pepped our cells after they had been left to grow for a further 24 hours. This was so we could send our constructs to iGEM headquarters and get them sequenced. Again we used the a <a href="https://www.thermofisher.com/order/catalog/product/K210010">ThermoFischer Purelink Quick Plasmid Miniprep kit</a>. We did have 9 samples (using 50 uL plate samples) but after a slight mishap, we were left with 8 samples.</p>
 +
 
 
</div>
 
</div>
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 11:42, 18 October 2016

September 5th 2016

We planned to mini prep our cell samples but Matt said that the IDT samples had not grown enough so we incubated them for another day. We then designed primers for PCR primer. In the afternoon we mini-prepped the DNA2.0 using a ThermoFischer Purelink Quick Plasmid Miniprep kit.

September 6th 2016

This morning we mini-pepped our cells after they had been left to grow for a further 24 hours. This was so we could send our constructs to iGEM headquarters and get them sequenced. Again we used the a ThermoFischer Purelink Quick Plasmid Miniprep kit. We did have 9 samples (using 50 uL plate samples) but after a slight mishap, we were left with 8 samples.