====Plasmids extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and BbaB0015====
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''By Sylvie''
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The OD were measured for each overnight cultures:
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{| class="wikitable center"
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|-
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!Sample
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!OD (1 ml)
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|-
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|FRB - GFP 11 in pSB1C3 (pPS16_019)
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|3
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|-
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|FKBP - GFP 10 in pSB1C3 (pPS16_018)
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|3.9
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|-
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|GFP 1.9 in pUC19 (pPS16_009)
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|4.2
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|-
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|BbaB0015
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|5
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|-
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|}
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Then the plasmids were extracted using a [[Team:Paris_Saclay/Experiments#Plasmid_DNA_extraction|"standard Plasmid Miniprep"]].
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====Digestion of extracted plasmids by EcoRI====
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''By Maxence & Caroline''
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Extracted pPS16_019, pPS16_018, pPS16_009 and BbaB0015 from the 10th October were digested by restriction enzymes EcoRI in order to verify the concentration as following:
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* 2 µL of plasmid
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* 2 µL of buffer FD
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* 2 µL of restriction enzyme EcoRI
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* 14 µL of water
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The mix were incubated for 1 hour at 37°C. Then, 20 µL of digestion products and 15 µL of DNA ladder were placed in wells and migrated at 100V during 30 min.
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[[File:T--Paris Saclay--Gelsylvie1.png|400px|thumb|center|Result of the migration]]
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The digestions were good for pPS16_019, pPS16_018 and BbaB0015, but we did not obtained the expecting band size for pPS16_009.
===BioBrick K2039000 and K2039001 characterization===
===BioBrick K2039000 and K2039001 characterization===
