Difference between revisions of "Team:Paris Saclay/Notebook/July/5"

(Created page with "{{Team:Paris_Saclay/notebook_header}} =Tuesday 5<sup>th</sup> July= ==Lab work== ===Visualization=== ====4,1 and 2,2 transformation==== The 04/06/2016 transformations show...")
 
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===Visualization===
 
===Visualization===
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====4,1 and 2,2 transformation====
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====Transformation of DH5α|pPS16_004 and DH5α|pPS16_007 ====
  
  
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4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)
 
4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)
  
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6 clones of each of the other transformations 1.1, 1.2, 2.1, 3.1 and 3.2 were placed on culture in 4mL of LB added to Ampicillin (50
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6 clones of each of the other transformations pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006 were placed on culture in 4mL of LB added to Ampicillin (50
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Protocol:
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Revision as of 15:25, 7 July 2016

Tuesday 5th July

Lab work

Visualization

Transformation of DH5α|pPS16_004 and DH5α|pPS16_007

The 04/06/2016 transformations show white colony growth for G-blocks 4.2 clone 3 and GFP1-9 (clone 1), but blue colonies only for 4.1 clone 3.

Thus this last G-block 4.1 clone 3 was transformed again following the same protocol as the 21/06/2016 with : 50μg of competent DH5α cells 4.1 ligation product in 5μL of the plasmid puc19 4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis)

6 clones of each of the other transformations pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006 were placed on culture in 4mL of LB added to Ampicillin (50