Difference between revisions of "Team:Virginia/Parts"

Revision as of 19:28, 18 October 2016

We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.

Bba_K1879000

This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al^refReference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. , and we included an RBS sequence upstream of the coding sequence.

Bba_K1879001

This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.

@@ Line 40: / Line 40: @@
 cleavage enzyme necessary to remove the CBZ protecting group from the <br>
 N-terminus of amino acids. The coding sequence was derived from Nanduri <br>
-et al (Ref), and we included an RBS sequence upstream of the coding sequence.</span></p>
+et al<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
+</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p>
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