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| − | <head> | + | <h1>Notebook:Ernesto Luna</h1> |
| − | <meta charset="utf-8"/> | + | <h2>LuxI Transformation</h2> |
| − | <title>LuxI Transformation · Benchling</title> | + | <h3>Thursday, 6/9/16</h3> |
| − | <link href="../benchling.css" rel="stylesheet"/> | + | <ol> |
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| + | <li>Gathered 2 amp agar plates from the cold room (4C) and warmed them up in the incubator at 37.C. </li> |
| − | <body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">LuxI Transformation</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Dates:</b> 2016-06-09 to 2016-06-14</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part58" data-index="0" style="margin-left:35px;">Gathered 2 amp agar plates from the cold room (4C) and warmed them up in the incubator at 37.C. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part59" data-index="1" style="margin-left:35px;">Prepared an ice bath to thaw out the frozen cells and maintain the plasmid DNA cold. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part60" data-index="2" style="margin-left:35px;">Gathered 1 tube of BL21 cells from -80C storage and placed in the ice bath to thaw (~10 min). </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>4.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part61" data-index="3" style="margin-left:35px;">Gathered "tube 2" of concentrated LuxI plasmid from the refrigirator and placed in the ice bath..</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>5.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part62" data-index="4" style="margin-left:35px;">Prepped 2 eppendorf tubes (1.5mL), one for the LuxI transformation, and another for a control. Mixed as follows and kept on ice for 10 mins.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part63" data-index="5" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part64-item" data-formattable="true" data-index="6" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="6" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part64-table" data-formattable="true" data-index="6" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part64-0-0" data-namespace="table-ns1379"><span>LuxI Transformation</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part64-0-1" data-namespace="table-ns1379"><span>LuxI Neg. (Control)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part64-1-0" data-namespace="table-ns1379"><span>8uL dH20 2uL plasmid 40uL BL21 cells</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part64-1-1" data-namespace="table-ns1379"><span>10uL dH20 40uL BL21 cells</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part64" data-index="6" style="width:265px;margin-left:0;">*Note: mixing procedure carried out on ice
| + | <li>Prepared an ice bath to thaw out the frozen cells and maintain the plasmid DNA cold. </li> |
| − | </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part65" data-index="7" style="margin-left:0;">6. Took warm agar plates (2) from the incubator and labeled with "plasmid (or negative control), cell strain, Abx, initials, date".</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part66" data-index="8" style="margin-left:0;">7. Pipetted entire volume of each mixture onto each respective plate. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part67" data-index="9" style="margin-left:0;">8. Added ~7-10 glass innoculation beads to each plate and swirled for a minute.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part68" data-index="10" style="margin-left:0;">9. Disposed of innoculation beads in flask containing EtOH. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part69" data-index="11" style="margin-left:0;">10. Placed plates upside down in the incubator and left over night. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part70" data-index="12" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div> | + | <li>Gathered 1 tube of BL21 cells from -80C storage and placed in the ice bath to thaw (~10 min). </li> |
| − | <div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">LuxI Transformation</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Dates:</b> 2016-06-09 to 2016-06-14</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/10/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part71" data-index="13" style="margin-left:0;">Followed up to check for growth. 3 small cultures grew in the LuxI plate and displayed a faint,red color. Disposed of negative control plate (no growth), and parafilmed LuxI plate to store in the cold room (4C). </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part72" data-index="14" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">LuxI Transformation</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Dates:</b> 2016-06-09 to 2016-06-14</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/14/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part73" data-index="15" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Receiver Transformation in DH5AT</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Date:</b> 2016-09-19</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part78" data-index="0" style="margin-left:0;">Transform DH5As with F2620, LuxR, GFP+, and negative rec. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part79" data-index="1" style="margin-left:35px;">Add 2uL of F2620, LuxR, and neg rec. and 1uL of GFP+ (very concentrated) to 30uL of DH5AT competent cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part80" data-index="2" style="margin-left:35px;">Incubate on ice for 10min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part81" data-index="3" style="margin-left:35px;">Heat shock at 42C for 35sec</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part82" data-index="4" style="margin-left:35px;">Incubate on ice for 2min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part83" data-index="5" style="margin-left:35px;">Add 750uL of SOC media (RT) to each tube</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part84" data-index="6" style="margin-left:35px;">Shake 37C for 30min</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part85" data-index="7" style="margin-left:35px;">Plate 100uL on LB+Amp</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>●</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part86" data-index="8" style="margin-left:35px;">Incubate overnight at 37C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part87" data-index="9" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Culture preparation for F2620 inductions</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Date:</b> 2016-09-26</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part92" data-index="0" style="margin-left:0;">Inoculated and grew cultures for each of the senders that I will be using to induce F2620. Due to well amount limitations on the 96 well plate, we are only inducing with 4 senders at a time. In this first run, we are only inducing with AubI, LuxI, LasI, and EsaI media. Below is a table of the 96 well plate organization (scroll to the sides to see everything). Intended to run the 8hr induction tomorrow, but I read Cassandra's protocol wrong and forgot to grow receiver cultures for seeding. Will be doing everything in triplicate and will use sender media concentrations of 10% and 50% in well volumes of 200uL. It is important to note that all senders are in Bl21 and all receivers are in DH5aT. </div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part93-item" data-formattable="true" data-index="1" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="1" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part93-table" data-formattable="true" data-index="1" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:1365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:1345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">H</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">I</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">J</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">K</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">L</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">M</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-0-0" data-namespace="table-ns1399"><span></span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-1" data-namespace="table-ns1399"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-2" data-namespace="table-ns1399"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-3" data-namespace="table-ns1399"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-4" data-namespace="table-ns1399"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-5" data-namespace="table-ns1399"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-6" data-namespace="table-ns1399"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-7" data-namespace="table-ns1399"><span>7</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-8" data-namespace="table-ns1399"><span>8</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-9" data-namespace="table-ns1399"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-10" data-namespace="table-ns1399"><span>10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-11" data-namespace="table-ns1399"><span>11</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part93-0-12" data-namespace="table-ns1399"><span>12</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-0" data-namespace="table-ns1399"><span>A</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-1" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>200uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-2" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>200uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-3" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>200uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-4" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL F2620 + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-5" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL F2620 + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-6" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL F2620 + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-7" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL GFP pos. + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-8" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL GFP pos. + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-9" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL GFP pos. + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-10" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL neg. rec. + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-11" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL neg. rec. + 50uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-1-12" data-namespace="table-ns1399" style="background-color:#FFE599;"><span>100uL neg. rec. + 50uL neg. sender + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-0" data-namespace="table-ns1399"><span>B</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-2-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 50uL LasI + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-0" data-namespace="table-ns1399"><span>C</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL F2620 + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL F2620 + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL F2620 + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-3-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL F2620 + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-0" data-namespace="table-ns1399"><span>D</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-4-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 50uL LasI + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-0" data-namespace="table-ns1399"><span>E</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL GFP pos. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL GFP pos. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL GFP pos. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-5-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL GFP pos. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-0" data-namespace="table-ns1399"><span>F</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec.+ 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec.+ 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec.+ 50uL AubI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec.+ 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec.+ 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec.+ 50uL LuxI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec.+ 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec.+ 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec.+ 50uL EsaI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec.+ 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec.+ 50uL LasI + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-6-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec.+ 50uL LasI + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-0" data-namespace="table-ns1399"><span>G</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-1" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-2" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-3" data-namespace="table-ns1399" style="background-color:#F7CAAC;"><span>100uL neg. rec. + 10uL AubI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-4" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-5" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-6" data-namespace="table-ns1399" style="background-color:#C5E0B3;"><span>100uL neg. rec. + 10uL LuxI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-7" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-8" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-9" data-namespace="table-ns1399" style="background-color:#BDD6EE;"><span>100uL neg. rec. + 10uL EsaI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-10" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-11" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-7-12" data-namespace="table-ns1399" style="background-color:#D9D9D9;"><span>100uL neg. rec. + 10uL LasI + 40 uL neg. sender + 50uL LB Amp</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-0" data-namespace="table-ns1399"><span>H</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-1" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-2" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-3" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-4" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-5" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-6" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-7" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-8" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-9" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-10" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-11" data-namespace="table-ns1399"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part93-8-12" data-namespace="table-ns1399"><span></span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part93" data-index="1" style="width:720px;margin-left:0;">
| + | <li>Gathered "tube 2" of concentrated LuxI plasmid from the refrigirator and placed in the ice bath.</li> |
| − | </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part94" data-index="2" style="margin-left:0;"><b><u>Sender Cultures:</u></b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part95" data-index="3" style="margin-left:0;">Inoculated 5, 15mL round bottom culture tubes with each respective sender. Grew at 37°C shaking overnight. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part96" data-index="4" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part97-item" data-formattable="true" data-index="5" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="5" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part97-table" data-formattable="true" data-index="5" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-0-0" data-namespace="table-ns1399"><span>Sender</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-0-1" data-namespace="table-ns1399"><span>Total media volume needed for run (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-0-2" data-namespace="table-ns1399"><span>Culture volume (mL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-1-0" data-namespace="table-ns1399"><span>Negative sender</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-1-1" data-namespace="table-ns1399"><span>1740</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-1-2" data-namespace="table-ns1399"><span>3</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-2-0" data-namespace="table-ns1399"><span>AubI</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-2-1" data-namespace="table-ns1399"><span>540</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-2-2" data-namespace="table-ns1399"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-3-0" data-namespace="table-ns1399"><span>LuxI</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-3-1" data-namespace="table-ns1399"><span>540</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-3-2" data-namespace="table-ns1399"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-4-0" data-namespace="table-ns1399"><span>EsaI</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-4-1" data-namespace="table-ns1399"><span>540</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-4-2" data-namespace="table-ns1399"><span>2</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part97-5-0" data-namespace="table-ns1399"><span>LasI</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-5-1" data-namespace="table-ns1399"><span>540</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part97-5-2" data-namespace="table-ns1399"><span>2</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table2</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part97" data-index="5" style="width:365px;margin-left:0;"> | + | <li>Prepped 2 eppendorf tubes (1.5mL), one for the LuxI transformation, and another for a control. Mixed as follows and kept on ice for 10 mins.</li> |
| − | </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part98" data-index="6" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">LB agar plate induction of F2620 using LuxI</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Date:</b> 2016-09-27</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part103" data-index="0" style="margin-left:0;"><b>Goal:</b> produce some qualitative data of an F2620 induction using LuxI. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part104" data-index="1" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part105" data-index="2" style="margin-left:0;">Inoculated 2 lb agar plates with LuxI sender and F2620 + receiver controls (LuxR, GFP+, neg. receiver). Inoculated by dabbing the agar plate after picking colonies from each source plate. Placed LuxI in the middle and each receiver at varying distances around it in a cross shape. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part106" data-index="3" style="margin-left:0;">Also set up a control plate with the same design, except I did not inoculate with LuxI. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part107" data-index="4" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part108" data-index="5" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part109" data-index="6" style="margin-left:0;">Also grew 5mL overnight cultures of LuxI, F2620, LuxR, GFP+, and negative receiver. Will inculate tomorrow in LB Amp plate with LuxI in the middle and F2620 and each control at varying distances from it in a cross shape. Will inoculate by pipetting drops of the overnight cultures in their respective positions (most likely 20uL LuxI and 5-10 uL of each receiver). Will also set up a control plate without LuxI in the middle. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part110" data-index="7" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">Sender media filtration and Receiver cultures</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Ernesto iGEM Notes</div><div><b>Authors:</b> Ernesto Luna</div><div><b>Date:</b> 2016-09-27</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part115" data-index="0" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part116" data-index="1" style="margin-left:0;"><b>Objective: </b>to filter out the HSL media from AubI, LuxI, LasI, and EsaI cultures to use for induction of F2620.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part117" data-index="2" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part118" data-index="3" style="margin-left:0;">Removed inoculation pipette tip from each culture tube and centrifuged in the large centrifuge for 10min @ 3.6x10 RPM. Extracted supernantant media with a pipette and then through the syringe with a 45um nylon filter (different syringe and filter for each collection). Collected all filtered media (~2-3mL each) in fresh, clean tubes and placed in 4°C room for use in induction tomorrow. Will have to rerun this part of the F2620 inductions since ideally we don't want the HSLs to wait too much since we don't know much about their stability. For now, will continue the inductions using this media, but will run again without waiting a day and compare. Hopefully, this yields quantitative insight on HSL stability based on OD GFP readings. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part119" data-index="4" style="margin-left:0;">-------------------------------------------------------------------------------------------------------------------------------------------</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part120" data-index="5" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part121" data-index="6" style="margin-left:0;">Since I didn't grow any reciever cultures yesterday, I grew them today. Inoculated 3, 15mL round bottom culture tubes with each respective receiver (F2620 and induction controls).</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part122" data-index="7" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part123-item" data-formattable="true" data-index="8" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="8" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part123-table" data-formattable="true" data-index="8" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part123-0-0" data-namespace="table-ns1415"><span>Receiver</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part123-0-1" data-namespace="table-ns1415"><span>Culture volume (mL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part123-1-0" data-namespace="table-ns1415"><span>F2620</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part123-1-1" data-namespace="table-ns1415"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part123-2-0" data-namespace="table-ns1415"><span>GFP +</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part123-2-1" data-namespace="table-ns1415"><span>5</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part123-3-0" data-namespace="table-ns1415"><span>Negative receiver</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part123-3-1" data-namespace="table-ns1415"><span>5</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part123" data-index="8" style="width:265px;margin-left:0;">
| + | <li>Took warm agar plates (2) from the incubator and labeled with "plasmid (or negative control), cell strain, Abx, initials, date".</li> |
| − | </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part124" data-index="9" style="margin-left:0;">These cultures will be used tomorrow to grow from a 10% seeding-fresh media ratio to OD 600. This will take approximately 5 hours. Once the cultures reach OD 600 (log phase growth), we will use them as the receiver volumes in the 96 well plate F2620 inductions. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part125" data-index="10" style="margin-left:0;"></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div></body> | + | <li>Pipetted entire volume of each mixture onto each respective plate. </li> |
| − | | + | <li>Added ~7-10 glass innoculation beads to each plate and swirled for a minute.</li> |
| | + | <li> Disposed of innoculation beads in flask containing EtOH. </li> |
| | + | <li>Placed plates upside down in the incubator and left over night. </li> |
| | + | </ol> |
| | + | <table> |
| | + | <th> |
| | + | <td></td> |
| | + | <td>A</td> |
| | + | <td>B</td> |
| | + | </th> |
| | + | <tr> |
| | + | <td>1</td> |
| | + | <td>LuxI Transformation</td> |
| | + | <td>LuxI Neg. (Control)</td> |
| | + | </tr> |
| | + | <tr> |
| | + | <td>2</td> |
| | + | <td>8uL dH20 2uL plasmid 40uL BL21 cells</td> |
| | + | <td>10uL dH20 40uL BL21 cells</td> |
| | + | </tr> |
| | + | </table> |
| | + | <h2>LuxI Transformation</h2> |
| | + | <h3>Friday, 6/10/16</h3> |
| | + | <p>Followed up to check for growth. 3 small cultures grew in the LuxI plate and displayed a faint,red color. Disposed of negative control plate (no growth), and parafilmed LuxI plate to store in the cold room (4C). </p> |
| | + | <h2>Receiver Transformation in DH5AT</h2> |
| | + | <h3>Monday, 9/19</h3> |
| | + | <p>Inoculated and grew cultures for each of the senders that I will be using to induce F2620. Due to well amount limitations on the 96 well plate, we are only inducing with 4 senders at a time. In this first run, we are only inducing with AubI, LuxI, LasI, and EsaI media. Below is a table of the 96 well plate organization (scroll to the sides to see everything). Intended to run the 8hr induction tomorrow, but I read Cassandra's protocol wrong and forgot to grow receiver cultures for seeding. Will be doing everything in triplicate and will use sender media concentrations of 10% and 50% in well volumes of 200uL. It is important to note that all senders are in Bl21 and all receivers are in DH5aT. </p> |
| | + | <img src="https://static.igem.org/mediawiki/2016/1/1f/T--Arizona_State--ernestotable1.png"> |
| | | | |
| | </html> | | </html> |
| | | | |
| | {{Arizona_State_Footer}} | | {{Arizona_State_Footer}} |
Followed up to check for growth. 3 small cultures grew in the LuxI plate and displayed a faint,red color. Disposed of negative control plate (no growth), and parafilmed LuxI plate to store in the cold room (4C).
Inoculated and grew cultures for each of the senders that I will be using to induce F2620. Due to well amount limitations on the 96 well plate, we are only inducing with 4 senders at a time. In this first run, we are only inducing with AubI, LuxI, LasI, and EsaI media. Below is a table of the 96 well plate organization (scroll to the sides to see everything). Intended to run the 8hr induction tomorrow, but I read Cassandra's protocol wrong and forgot to grow receiver cultures for seeding. Will be doing everything in triplicate and will use sender media concentrations of 10% and 50% in well volumes of 200uL. It is important to note that all senders are in Bl21 and all receivers are in DH5aT.
