Difference between revisions of "Team:Virginia/Parts"
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| + | <p><span class="p"> | ||
| + | We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) | ||
| + | cleavage enzyme part, and a composite part consisting of a promoter, the CBZ | ||
| + | cleavage enzyme, and a terminator.</span></p> | ||
| + | |||
| + | <span><img style="height:310px; width: 280px; float:right" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png"/> | ||
| + | <br><i></i></span> | ||
| + | <br><br><br> | ||
| + | <span class="ptitle">Bba_K1879000</span><br><br> | ||
| + | <p><span class="p"> | ||
| + | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) <br> | ||
| + | cleavage enzyme necessary to remove the CBZ protecting group from the <br> | ||
| + | N-terminus of amino acids. The coding sequence was derived from Nanduri <br> | ||
| + | et al<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. | ||
| + | </span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> | ||
| + | |||
| + | <br><br><br><br><br><br><br><br><br><br><br><br> | ||
| + | <span><img style="height:310px; width: 280px; float:right" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png"/> | ||
| + | <br><i></i></span> | ||
| + | |||
| + | <br><br><span class="ptitle">Bba_K1879001</span><br><br> | ||
| + | |||
| + | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage<br> | ||
| + | enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our <br> | ||
| + | biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce<br> | ||
| + | wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively <br> | ||
| + | cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | ||
| + | </span></p> | ||
Revision as of 02:10, 19 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et alrefReference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
, and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
