Difference between revisions of "Team:Virginia/Parts"
| Line 76: | Line 76: | ||
<br><br><br><br> | <br><br><br><br> | ||
<span class="ptitle">Bba_K1879001</span><br><br> | <span class="ptitle">Bba_K1879001</span><br><br> | ||
| − | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage | + | <p><span class="p">This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage |
| − | enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our | + | enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our |
| − | biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce | + | biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce |
| − | wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively | + | wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively |
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. | ||
</span></p> | </span></p> | ||
Revision as of 02:13, 19 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et alrefReference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
, and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
