The UT Austin iGEM Team would love to thank everyone who has helped us achieve our goals, no matter how big or small the contribution. Without these acts of generosity, we would have not accomplished all that we have done.

The following labs, professors, post docs, and graduate students were instrumental in helping us learn specific techniques, sharing reagents or strains or equipment with us, or for giving input that influenced our project

• Dr. Chris Yellman and [http://www.marcottelab.org/index.php/Main_Page/ The Marcotte Lab] for providing ADH and PDC S. cerevisiae knockouts.
• Aziz Al'Khafaji and [http://www.brocklab.com/ The Brock Lab] for providing a fluorescence microscope and advice on improving conjugation experiments.
• Jiri Perutka and [http://ellingtonlab.org The Ellington Lab] for constructing the plasmids pBTK518, pBTK19 and pBTK520 which we used for conjugation.
• [http://barricklab.org/twiki/bin/view/Lab/ The Barrick Lab] for providing helpful advice and comments throughout the summer.

The following students either assisted us as we learned new techniques, began our project, gave feedback on an array of things, or helped us keep the lab neat, clean, and well stocked.

• Kimberly Ornelas for aiding us with 16s sequencing, conjugation and recapitulation.
• Samah Khan for helping us initially isolate various microbes from different kombucha brands.
• Natalie Schulte and Samita Charolia for their initial research on pH sensitive promoters for use in E. coli and creating the original experimental procedures for general pH testing.
• Nadia Zaidi and Jocelyn Li for
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• Kelsey Hu for working throughout the summer to keep the lab stocked with media and for providing assistance running pH experiments.
• Riya Sreenivasan for creating the amilCP construct for the pH project controls.

The following iGEM students worked on specific aspects of our project.

• Matthew Hooper for identifying and sequencing bacteria and yeast strains in store bought kombucha, recapitulating the strains, and screening for ethanol levels.
• Zachary Martinez for initial kombucha strain identification their conjugations.
• Katelyn Corley for making recapitulations from the previously identified kombucha strains.
• Ian Overman and Alexandra Alario for working with and characterizing the pH sensors CadC, P-atp2, and G. oxydans.
• Sofia Chinea for working with and characterizing the pH sensor CpxR, and for identifying potential pH sensitive promoters in G. oxydans.
• Prachi Shah for the work done on Brazen.
• Stratton Georgoulis for ethanol production assays.

All iGEM team members collaborated on the wiki.

Greg Goodman and KTonic for giving us a clear direction in how to expand and progress our current work with kombucha, while keeping in mind issues of intellectual property, the product and what the consumer wants in their product, and how bench science can be vital to many things beyond the bench.

ALSO: Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.

Funding

• BEACON
• NSF CAREER (CBET-1554179)